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Method for extracting coumarin compounds from morinda officinalis

A technology for coumarins and compounds, which is applied in the field of compound extraction and analysis, can solve the problem of no detection method for coumarin compounds, and achieves the effects of saving analysis time, saving organic solvents, and high sensitivity

Pending Publication Date: 2022-01-07
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there is no detection method for coumarin compounds in Morinda officinalis, and for the detection of coumarin compounds, the key step is sample pretreatment

Method used

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  • Method for extracting coumarin compounds from morinda officinalis
  • Method for extracting coumarin compounds from morinda officinalis
  • Method for extracting coumarin compounds from morinda officinalis

Examples

Experimental program
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Embodiment 1

[0031] The determination of the extraction method and control parameters of coumarin compounds in Morinda officinalis in embodiment 1

[0032] 1. The extraction method of coumarin compounds in Morinda officinalis comprises the following steps,

[0033] S1: adjust the pH of Morinda officinalis extract to 1-7;

[0034] S2: adding extractant (dissolved in dispersant) to obtain mixed liquid;

[0035] S3: After the mixed liquid is treated at 10-60°C, ultrasonic-assisted extraction is performed;

[0036] S4: Centrifuge the mixed liquid after ultrasonic treatment to obtain sediment and complete the extraction.

[0037] 2. Determination of control parameters

[0038] The main factors affecting the efficiency of ultrasonic-assisted extraction and dispersion liquid-liquid microextraction include: type and amount of extractant, extraction temperature, type and amount of dispersant, as well as ionic strength and pH.

[0039] 2.1. Determination of the type of ultrasonic-assisted extrac...

Embodiment 2

[0046] The determination of coumarin compound in embodiment 2 Morinda officinalis sample

[0047] 1. Preparation of standard solution: single standard stock solution: precisely weighed coumarin, dihydrocoumarin, acenocoumarin, 6-methylcoumarin, 7-hydroxy-6-methoxycoumarin Each of 2.15mg, 1.25mg, 1.30mg, 1.40mg, 1.30mg was dissolved in 50% methanol, and the volume was adjusted to 10mL respectively, and the concentrations were respectively 215μg / mL, 125μg / mL, 130μg / mL, 140μg / mL, 130μg / mL single standard stock solution. Mixed standard solution: Precisely draw an appropriate amount of each single standard stock solution, and prepare a mixed standard solution with a corresponding concentration gradient.

[0048] 2. Sample pretreatment: Grind the sample of Morinda officinalis into fine powder, take 100g of Morinda officinalis fine powder, put it in a 3000mL stainless steel pot, add 1200mL of distilled water, boil for 2 hours, and then add 1000mL of distilled water to the filtered re...

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Abstract

The invention belongs to the technical field of compound extraction and analysis, and particularly relates to a method for extracting coumarin compounds from morinda officinalis. The method comprises the following steps of: adjusting the pH value of morinda officinalis extract to 1-7; adding trichloromethane to obtain a mixed liquid; treating the mixed liquid under the condition of 10-60 DEG C, and then carrying out ultrasonic-assisted extraction; and centrifuging the mixed liquid subjected to ultrasonic treatment to obtain a precipitate, thereby completing extraction. According to the method, the high performance liquid chromatography is adopted, the sensitivity of measuring five coumarin compounds in morinda officinalis is high, and the detection limit is 0.5-0.9 ng / mL; and the used extraction solvent is less, the consumed time is short, and the method can be used for determining coumarin compounds in a large batch of morinda officinalis samples.

Description

technical field [0001] The invention belongs to the technical field of compound extraction and analysis, and specifically relates to an extraction method of coumarin compounds in Morinda officinalis, which is a method for extracting and analyzing coumarin compounds in Morinda officinalis based on ultrasonic-assisted dispersion liquid-liquid microextraction . Background technique [0002] Morinda officinalis contains coumarin components, and its adverse reactions are mostly related to oral administration. Some studies have found that LD mice administered with coumarin 50 =1.39g / kg, strong toxicity, although this toxicity may have species differences, and whether coumarin causes poisoning is related to the polymorphism of CYP2A6 enzyme in the population. [0003] At present, there is no detection method for coumarin compounds in Morinda officinalis, and for the detection of coumarin compounds, the key step is sample pretreatment. After the pretreatment of the sample, on the ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/89
CPCG01N30/06G01N30/89
Inventor 夏小乐陆智刘凤松裴孝梅龙梦飞
Owner JIANGNAN UNIV
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