Application of zinc gluconate in preparation of Bacteroides regulator
A technology of zinc gluconate and Bacteroides, which is applied in the field of medicine to achieve the effect of increasing abundance and promoting mass proliferation
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[0018] Since the intestinal flora derived from the human body cannot completely colonize the mice, human feces were taken as intestinal flora samples, and in vivo simulation experiments were carried out in vitro to observe the effect of zinc gluconate intervention on Bacteroides Adjustment, the specific operation process is as follows:
[0019] 1. Fecal sample processing
[0020] (1) PBS buffer preparation
[0021] Taking 1000ml as an example, weigh the following medicines with an electronic analytical balance and make records, and pour them into a 1000ml beaker, including: KH 2 PO 4 0.24g, Na 2 HPO 4 12H 2 O 2.90g, NaCl 8.00g, KCl 0.20g.
[0022] Use a graduated cylinder to measure 800ml of ultra-pure water and pour it into a beaker, put it into the stirring rotor, and put it on a magnetic heating stirrer to stir until it is completely dissolved, then use a calibrated pH meter to measure the pH of the above solution, use 0.1M HCl or NaOH adjusted pH=7.4±0.05.
[0023...
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