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Microbial carbon-based fertilizer as well as preparation method and application thereof
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A biochar and microbial technology, applied in the field of fertilizers, can solve the problems of low colonization rate, poor activity and inability to form microbial strains
Pending Publication Date: 2022-01-14
NORTHWEST A & F UNIV
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Although biochar can benefit soil health by enhancing infiltration, retaining water and nutrients, and improving fertility, biochar alone cannot form a complete fertilizer to provide rich nutrients for crops, and a better choice is biochar as a carrier Combine with functional microbial strains to establish multiple linkages or interactions to improve the efficiency of functional microbes
[0004] In view of this, the development of microbial charcoal-based fertilizer of Bacillus amyloliquefaciens B140, and its growth-promoting effect on cucumber and the evaluation of the control effect of cucumber wilt, aim to further optimize the disease prevention and growth-promoting ability of Bacillus amyloliquefaciens B1408 strain , in order to solve the problems of the current disease-prevention and growth-promoting strains and the low colonization rate and poor activity of microbial strains after the application of existing microbial fertilizers
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Embodiment 1
[0045] The preparation of embodiment 1 bacillus amyloliquefaciens B1408 seed liquid and bacterial suspension
[0046] The activated strain B1408 was inoculated into LB medium, and cultured on a constant temperature shaker at 28°C, 170r / min for 12h to obtain a seed liquid, and then inoculated with 2% inoculum in 100mL LB medium, at 28°C, 170r / min / min cultured for 48h. Then, after centrifuging at 8000r / min for 10min, the precipitated cells were collected, the harvested cells were resuspended in sterile water, and the concentration of the bacterial solution was adjusted to 10 8 CFU / mL to prepare the bacterial suspension.
Embodiment 2
[0047] Example 2 Three kinds of biochar neutral treatments and their effects on the growth of Bacillus amyloliquefaciens B1408
[0048] Soak the three kinds of biochar with 0.1mol / L HCl overnight, then rinse repeatedly with deionized water until the pH is neutral, filter with suction, and dry to make neutral wheat straw biochar and rice straw biochar respectively , corn stalk biochar, respectively recorded as WSB (neutral), RSB (neutral), CSB (neutral).
[0049] Weigh 0.1 g each of the three biochars with natural pH and the three biochars after neutral treatment, and sterilize them at 121°C for 25 minutes with moist heat.
[0050] Put 100mL of LB medium into a 250mL Erlenmeyer flask, sterilize it at 121°C for 25 minutes, and inoculate the prepared B1408 seed solution after cooling, the inoculation amount is 2% of the medium. At the same time, the sterilized biochar was inserted into the medium, and cultured at 28° C., 170 rpm / min for 60 h. Samples were taken at different tim...
Embodiment 3
[0052] The condition optimization of embodiment 3 biochar adsorption Bacillus amyloliquefaciens B1408
[0053] (1) Effects of different types of biochar on the adsorption of B1408 bacteria
[0054] Select 100-120 mesh wheat straw biochar, rice straw biochar, and corn straw biochar to screen for biochar types.
[0055] (2) Effect of different particle sizes of biochar on biochar adsorption
[0056] On the basis of the biochar with the best adsorption capacity obtained in (1), three different particle size ranges of 40-60 mesh, 100-120 mesh, and 180-200 mesh were screened.
[0057] (3) Effect of adsorption time on biochar adsorption
[0058] On the basis of the best adsorption capacity in (1) and the best adsorption particle size in (2), the biochar was screened for different adsorption times of 6, 12, 24, 48, 60 and 72 h.
[0059] (4) Effect of oscillation speed on biochar adsorption
[0060] On the basis of (1) the best adsorption capacity, (2) the best particle size and (...
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Abstract
The invention discloses a microbial carbon-based fertilizer as well as a preparation method and application thereof. Wheat strawbiochar and rice hullbiochar are screened as optimal biochar with the influence of biochar on the growth of bacillus amyloliquefaciens as an index, and the physicochemical properties of the biochar are measured. It is discovered through further condition optimization that the optimal particle size of adsorption of the two kinds of biochar to bacillus amyloliquefaciens B1408 (CGMCC No.15110) is 180-200 meshes, the optimal adsorption time is 48 hours, the optimal oscillation speed is 170 rpm / min, and the optimal ratio of the bacterial suspension to the biochar is 20: 1. Then, two technological processes of adsorption and blending are adopted for exploration, and four bacillus amyloliquefaciens B1408 microbial carbon-based fertilizers including wheat charcoal adsorption B1408 (WSBX), wheat charcoal blending (WSBC), rice charcoal adsorption (RSBX) and rice charcoal blending (RSBC) are prepared. Finally, it is found that the four microbial carbon-based fertilizers have good effects on cucumber growth promotion and cucumber fusarium wilt prevention and control, and have good development and application prospects.
Description
technical field [0001] The invention belongs to the technical field of fertilizers, and in particular relates to a microbial carbon-based fertilizer, a preparation method and an application thereof. Background technique [0002] As important means of agricultural production, chemical fertilizers and pesticides have played an important role in ensuring national food security. However, problems such as the quality and safety of agricultural products and environmental pollution caused by a large number of irrational use have become increasingly serious. The reduction of chemical fertilizers and pesticides and the increase in efficiency have become the key to promoting high-quality agriculture. A national strategy for development. Microbial pesticides (fertilizers) have good environmental compatibility, and have unique advantages in promoting the reduction of chemical fertilizers and pesticides, increasing their efficiency, and promoting plant health, and their product research ...
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