Industrial fast test tube reproduction method for African chrysanthemum

A rapid propagation and test-tube technology, applied in gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve problems such as inability to meet factory production, and achieve the effects of fast reproduction, considerable economic benefits and high efficiency

Inactive Publication Date: 2004-02-25
BEIJING JINXIU DADI AGRI CO LTD
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  • Abstract
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Problems solved by technology

[0003] The object of the present invention is to aim at the above-mentioned defects that cannot satisfy the industrial production in the prior art, and provide a factory test-tube rapid propagation method suitable for the rapid propagation of gerbera. The method has the advantages of fast propagation speed, suitable for large-scale production, and stable properties. Features

Method used

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Embodiment Construction

[0024] The following are embodiments of the present invention:

[0025] 1. The rapid propagation process of tissue culture test-tube plantlets:

[0026] 1. Collection of explants: choose gerbera flower buds with a diameter of 0.3 to 1 cm (the pistils are wrapped by sepals) as culture materials, because the basal flowers of the leaves are located near the soil in the leaf axils at the base of the leaves. If you are not careful, it is easy to pollute the dirt, and it is not easy to disinfect. You can add an appropriate bag cover to keep the buds clean before collecting.

[0027] 2. Material cleaning and disinfection treatment: the freshly collected explants were rinsed with running water, aseptic disinfection: sterilized with 75% medical alcohol for one minute, then sterilized with 0.1% bromogeramine for 10 minutes, and then continued to sterilize with 0.1% mercuric chloride For 5 minutes, rinse with sterile water 4 to 5 times, and dry the moisture on the surface of the materia...

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Abstract

Test tube African Chrysanthemum seedling, obtained by using young bud as explant and through dedifferentiation and re-differentiation induction and tissue culture, is transition cultured first in 100 % vermiculite medium for one month and then in other medium for 3-4 months to obtain 10-12 cm high commercial plant with 5-6 leaves. The plant may bloom after more half an year. The method can obtain progeny with stable gentic character, may be used in all seasons for industry production of African chrysanthemum.

Description

technical field [0001] The invention relates to a method for rapidly propagating gerbera miniature seedlings in test tubes. Background technique [0002] Gerbera, also known as gerbera, is a flower with great ornamental value. There are many varieties of cut gardens, including white, orange, red, yellow, pink and other colors. The patent of gerbera tissue culture and factory seedling raising technology. Conventional propagation methods include division propagation and sowing propagation. The slow speed is not conducive to large-scale production, and sowing propagation is easy to produce trait separation. Although "Plant Physiology Letters" Volume 35 No. 3 "Tissue Culture and Rapid Propagation of Gerbera" in June, 1999 disclosed the propagation process of Gerbera tissue culture test-tube plantlets and the transitional culture process of test-tube plantlets, but due to lack of sufficient It is difficult to guarantee the survival rate of seedlings and the rooting rate of test...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李荣旗刘蕾王玉忠林翔鹰王敏
Owner BEIJING JINXIU DADI AGRI CO LTD
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