Method for simultaneously detecting multiple components in multiple traditional Chinese medicine extracts by using UPLC-PDA
An extract and multi-component technology, which is applied in the field of traditional Chinese medicine detection, can solve the problem that the standard method for Echinacea purpurea extract and dandelion extract is not included, and achieves the effects of saving reagents, strong specificity and improving work efficiency.
Active Publication Date: 2022-01-14
山东省饲料兽药质量检验中心 +1
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Problems solved by technology
Neither the 2020 Pharmacopoeia nor the 2020 Veterinary Pharmacopoeia recorded the standard methods for extracts of Echinacea purpurea, honeysuckle and dandelion
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[0037] A detection method for simultaneously determining 7 components in 3 kinds of traditional Chinese medicine extracts using a UPLC-PDA detector. The 3 kinds of Chinese medicine extracts are echinacea extract, dandelion extract, and honeysuckle extract. Chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 4,5-di-O-caffeoylquinic acid, cichoric acid, monocaffeoyl tartaric acid, and caffeic acid.
[0038] Specifically include the following steps:
[0039] Step 1: Preparation of the test solution: take the test product, add different concentrations of methanol or ethanol water, ultrasonically extract for 25-35 minutes, centrifuge at 10000rpm / min for 5 minutes, filter the supernatant and measure it on the machine;
[0040] The second step, the preparation of the reference substance solution: respectively take chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 4,5-di-O-caffeoylquinic acid, cichoric acid, monocaffeoyl tartaric acid, and caffeic acid control ...
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The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for simultaneously detecting multiple components in multiple traditional Chinese medicine extracts by using UPLC-PDA, which comprises the following steps: preparing a test sample solution: taking a test sample, adding a solvent, carrying out ultrasonic extraction, centrifuging and filtering to obtain supernate, namely a test sample solution, for later use; preparing a reference substance stock solution: respectively taking each component to be detected as a reference substance, and adding a solvent to prepare a reference substance stock solution for later use; performing UPLC-PDA detection: respectively taking the test sample solution obtained in the step 1 and the reference substance stock solutions obtained in the step 2, injecting the test sample solution and the reference substance stock solutions into an ultra-high performance liquid chromatograph and a PDA detector, and performing gradient elution to respectively obtain chromatograms and spectrograms of the test sample and the reference substances; and performing result determination: carrying out comparative analysis on the spectrograms and chromatograms of the test sample and the reference substances, and carrying out qualitative identification and quantitative determination on each component.
Description
technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, in particular to a method for simultaneously detecting multiple components in various traditional Chinese medicine extracts by using UPLC-PDA. Background technique [0002] In the animal husbandry industry, with the growing development and production of traditional Chinese veterinary medicines, in the production process of traditional Chinese veterinary medicines, Chinese medicine extracts, as an intermediate product, urgently need quality control to finally control the quality of the final product. Neither the 2020 Pharmacopoeia nor the 2020 Veterinary Pharmacopoeia recorded the standard methods for Echinacea purpurea extract, honeysuckle extract and dandelion extract. The present invention intends to provide a research basis for the formulation of national quality standards for the three extracts in the future. Contents of the invention [0003] In order to sol...
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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N30/8679G01N2030/062G01N2030/065G01N2030/3007G01N2030/324Y02A50/30
Inventor 魏秀丽邹明王海挺张传津张志民刘明强
Owner 山东省饲料兽药质量检验中心