Preparation method of fresh dendrobium aqueous extract for treating cell injury and inflammation

A cell damage and fresh dendrobium technology is applied in the field of preparation of fresh dendrobium water extracts for the treatment of cell damage and inflammation, which can solve the problems such as cell damage and inflammation of dendrobium that have not yet been seen, and achieves inhibition of migration growth or proliferation and differentiation, high social benefits and high social efficiency. Economic benefits and high degree of industrialization

Inactive Publication Date: 2022-01-18
安徽同济生生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the use of Dendrobium for cell injury and inflammation.

Method used

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  • Preparation method of fresh dendrobium aqueous extract for treating cell injury and inflammation
  • Preparation method of fresh dendrobium aqueous extract for treating cell injury and inflammation
  • Preparation method of fresh dendrobium aqueous extract for treating cell injury and inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of fresh dendrobium water extract preparation method for the treatment of cell damage and inflammation, such as figure 1 shown, including the following steps:

[0028] S1: Take 100g of fresh dendrobium, remove the roots and leaves, keep the stem, and crush through a 100-mesh sieve;

[0029] S2: add 550ml of distilled water to immerse the medicinal materials, and repeat the extraction twice in a 75°C water bath, each time for 1h;

[0030] S3: Collect multiple filtrates and combine them, and use filter membrane to filter and sterilize to obtain the extract;

[0031] S4: obtained by concentrating the fresh dendrobium extract under reduced pressure at 45°C to 1 / 6-1 / 8 of the original volume, and then freeze-drying to constant weight.

[0032] The fresh Dendrobium is one or both of Dendrobium Huoshanense, Dendrobium candidum, Dendrobium nobile, Dendrobium fringe and Dendrobium drumstick, Dendrobium primula, Dendrobium spp. more than one combination.

[0033] The fr...

Embodiment 2

[0038] A kind of fresh dendrobium water extract preparation method for the treatment of cell damage and inflammation, such as figure 1 shown, including the following steps:

[0039] S1: Take 100g of fresh dendrobium, remove the roots and leaves, keep the stem, and crush through a 100-mesh sieve;

[0040] S2: add 500ml of distilled water to immerse the medicinal materials, and repeat the extraction 3 times in a water bath at 80°C, 1 hour each time;

[0041] S3: Collect multiple filtrates and combine them, and use filter membrane to filter and sterilize to obtain the extract;

[0042] S4: obtained by concentrating the fresh dendrobium extract under reduced pressure at 55° C. to 1 / 6-1 / 8 of the original volume, and then freeze-drying to constant weight.

Embodiment 3

[0044] A kind of fresh dendrobium water extract preparation method for the treatment of cell damage and inflammation, such as figure 1 shown, including the following steps:

[0045] S1: Take 100g of fresh dendrobium, remove the roots and leaves, keep the stem, and crush through a 100-mesh sieve;

[0046] S2: add 700ml of distilled water to immerse the medicinal materials, and repeat the extraction twice in a water bath at 85°C, each time for 1h;

[0047] S3: Collect multiple filtrates and combine them, and use filter membrane to filter and sterilize to obtain the extract;

[0048] S4: obtained by concentrating the fresh dendrobium extract under reduced pressure at 45°C to 1 / 6-1 / 8 of the original volume, and then freeze-drying to constant weight.

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Abstract

The invention discloses a preparation method of a fresh dendrobium aqueous extract for treating cell injury and inflammation, and relates to the technical field of pharmacy. The invention aims to solve the problem of inflammation curative effect caused by cell injury of dendrobium. The method specifically comprises the following steps: taking 100g of fresh dendrobium, removing roots and leaves, reserving stems, crushing, and sieving with a 100-mesh sieve; adding 400-800ml of distilled water to immerse the medicinal materials, and repeatedly extracting for 2-4 times in a water bath of 70-85 DEG C, 1 hour each time; collecting multiple filtrates, combining, and filtering and sterilizing by using a filter membrane to obtain an extracting solution; concentrating a fresh dendrobium extracting solution at 40-55 DEG C under reduced pressure until the volume is 1 / 6-1 / 8 of the original volume, and then freeze-drying until the weight is constant. The fresh dendrobium is dendrobium huoshanense, dendrobium officinale, dendrobium nobile, dendrobium fimbriatum, dendrobium chrysotoxum, dendrobium aphyllum and dendrobium primulina. The fresh dendrobium aqueous extract has a certain protection effect on treatment of cell injury, and can inhibit inflammatory response induced by LPS..

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to a method for preparing fresh dendrobium water extract for treating cell damage and inflammation. Background technique [0002] Trauma is an important disease that affects the health of the body. All skin trauma involving the dermis will end in the formation of scar tissue. Excessive hyperplasia of scar tissue often destroys the integrity of the human body surface, which not only affects the appearance, but also seriously affects the body. normal function. Fibroblasts are the main effector cells of scar tissue formation. Their activation, proliferation, migration, collagen synthesis and abnormal differentiation directly lead to the formation of scar tissue. At present, there are many kinds of drugs for treating scar formation, but the therapeutic effect is mediocre, and they have toxic and side effects, so their clinical application is restricted. The fresh or dried stems of va...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61P17/02A61K135/00
CPCA61K36/8984A61P17/02A61K2236/331A61K2236/53A61K2236/51
Inventor 余茂耘韩际宏段亚君梁英权杨潇潇陈元利张丰陈贵兰
Owner 安徽同济生生物科技有限公司
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