The application of rca gene of Orchid variegata and its vector construction method

Abstract

The invention belongs to the technical field of plant genetic engineering, and specifically discloses the application of the RCA gene of the variegated orchid and a vector construction method thereof. The RCA gene can be expressed normally by transferring the RCA of the variegated orchid into Dendrobium officinale, and the overexpression of the RCA gene can increase the transgene The polysaccharide content in Dendrobium officinale, the nucleotide sequence of the RCA gene is shown in SEQ ID NO: 1, and the overexpression of the RCA gene can greatly improve the protein content, the soluble sugar content and the photosynthetic pigment content in the transgenic Dendrobium officinale. The chloroplasts were well developed when increased, while the knockout protocorms could not develop normally and had no chloroplasts.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and particularly relates to the application of the RCA gene of Spotted orchid and a method for constructing a vector thereof. Background technique [0002] China is very rich in wild orchids, but all wild orchids are listed as endangered plants, and people's demand for orchids with unique leaf shapes, flower shapes and colors is increasing. After a long-term selection and cultivation of orchids, there have been many variant varieties. According to the different ornamental parts, they are divided into two types: flower art and leaf art. Among them, the leaf spot art orchid originated in China and has gradually been recognized by people in recent years. . The so-called leaf art refers to the relatively stable variation of the characters on the leaves of orchids, including changes in leaf color and leaf shape. The traditional leaf orchid mainly changes the color of the leaves, an...

AI Technical Summary

Problems solved by technology

[0003] Rubisco (ribulose-1,5-bisphosphate carboxylase / oxygenase) is abundant in the leaves of plants and plays an important role in regulating plant pigments, photosynthetic carbon assimilation and photorespiration. Among them, yellow or white striped stripes of different lengths and thicknesses appear on the leaves, and the gene presents a down-regulated expression form, and existing studies have shown that the expression of Rubisco activity depends on the regulation of RCA, in the role of RCA Rubisco can only show carboxylation or oxygenation activity. The exploration and application of the RCA (Rubisco activase) gene that regulates the expression of Rubisco gene in Orchid japonica has become a research direction, and there are no related reports so far.

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