Preparation method and application of dental pulp and dentin complex

A technology of dentin and composites, applied in the field of medical devices, to promote the migration of stem cells, accelerate the healing process, and promote the regeneration of dental pulp

Active Publication Date: 2022-01-28
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the release and degradation trend of growth factors in liquid-phase concentrated growth factors (LPCGF) need further research

Method used

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  • Preparation method and application of dental pulp and dentin complex
  • Preparation method and application of dental pulp and dentin complex
  • Preparation method and application of dental pulp and dentin complex

Examples

Experimental program
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Embodiment 1

[0042] 1. Primary and subculture of dental pulp cells

[0043] S1 Separation and culture: Collect healthy, complete, caries-free, and crack-free wisdom teeth from patients aged 18-22 years old who were treated in the Oral Surgery Clinic of West China Stomatological Hospital of Sichuan University due to impacted or orthodontic extraction. Informed consent was obtained from the patients and their families . Immediately after being completely pulled out, it was placed in pre-cooled PBS containing 10% penicillin-streptomycin double-antibody, and the ice box was refrigerated and quickly brought to the laboratory. The isolated teeth were rinsed three times with PBS containing 1% PS, and split with a hammer under sterile conditions. Take out the pulp tissue in the ultra-clean bench and cut it into pieces, put it into PBS containing 1% PS, then put it into a centrifuge tube and centrifuge at 1100 rpm for 5 minutes, discard the supernatant after centrifugation Add 1mL of 3mg / mL type ...

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Abstract

The invention relates to a preparation method and application of a dental pulp and dentin complex. The preparation method of the dental pulp and dentin complex comprises the following steps of: primary culture and subculture of dental pulp cells; preparing a liquid-phase concentrated growth factor; and mixing the cultured dental pulp cells with the liquid-phase concentrated growth factor to obtain a mixed material. Therefore, the dental pulp and dentin complex structure is prepared, and regeneration of dental pulp tissue is effectively promoted. According to the preparation method and application of the dental pulp and dentin complex of the invention, the autologous dental pulp cells and liquid-phase concentrated growth factor are mixed to be used as a young permanent tooth pulp tissue engineering scaffold, so that continuous growth of tooth roots can be promoted, and pains of patients can be relieved; the non-immune material is taken, the ectopic dental pulp-like tissue can be generated by transplantation of the dental pulp stem cells, the quantity of transplanted cells can be controlled, cell subgroups with the best potential effect on regeneration of the dental pulp and dentin complex can be screened, dental pulp regeneration is promoted, and dental pulp activity is recovered; and the liquid-phase growth factor can accelerate the wound healing process, and tissue regeneration is facilitated by stimulating cell proliferation and differentiation and promoting stem cell migration.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a preparation method and application of a pulp-dentine complex. Background technique [0002] At present, tissue engineering method is an ideal technical means to realize pulp tissue regeneration in the future, which provides a new direction for the treatment of dental pulp diseases. Dentin-pulp complex has the potential to secrete dentin matrix, maintain tooth vitality, repair itself, provide nutrition to tooth hard tissue and form dentin, and resist external damage. [0003] Therefore, finding a way to regenerate dentin-pulp complex to replace traditional root canal treatment and restore tooth vitality and function has become the development direction of scientific research and clinical practice in recent years. [0004] The research on the construction of pulp-dentin complex is the key to the study of pulp tissue regeneration. Although people have invested a lot of re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/54C12N5/077
CPCA61L27/3804A61L27/3895A61L27/54C12N5/0654A61L2430/12A61L2300/414A61L2300/602C12N2509/10C12N2509/00
Inventor 高波李雯旭叶玲余思静刘柯玥王婧宜
Owner SICHUAN UNIV
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