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Production method of biocontrol urea containing bacillus subtilis

A kind of Bacillus subtilis, production method technology, applied in the field of new fertilizer

Pending Publication Date: 2022-01-28
SHANDONG RUNYIN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at problems such as market gaps existing in the prior art, the present invention provides a production method containing Bacillus subtilis biocontrol urea. The present invention aims at the current farmers' fertilization habits and the accumulation of pathogenic bacteria in soil, according to the physiological conditions of Bacillus subtilis characteristics, prepared a new type of biocontrol urea

Method used

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  • Production method of biocontrol urea containing bacillus subtilis
  • Production method of biocontrol urea containing bacillus subtilis

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Embodiment 1

[0025] (1) Bacillus subtilis grows and reproduces particularly rapidly in liquid medium. Spread the pure Bacillus subtilis liquid on 6 agar plates respectively, draw lines in a mesh shape, place in a constant temperature incubator, and culture at a constant temperature of 56°C for 24 hours , colonies formed on the surface of the culture medium, and a single colony with good growth was selected from 6 plates, picked up with an inoculation loop and transplanted into a shaker containing 25ml of liquid medium, placed in a shaker, and oscillated at a constant temperature of 56°C for 24 hours The excellent strain solution was obtained, and then coated on 6 agar plates, cultured at a constant temperature of 56°C for 24 hours, and a single colony with good growth was selected from the 6 plates, transplanted into a liquid medium, and cultured at a constant temperature of 56°C for 24 hours, and the second generation of excellent bacteria was obtained. Bacterial strains were spread on 6 a...

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Abstract

The invention belongs to the technical field of novel fertilizers, and directly relates to a production method of biocontrol urea containing bacillus subtilis. The production method comprises the following steps: firstly, culturing three generations of excellent strains, and then carrying out seed culture to obtain a bacillus subtilis fermentation seed solution; inoculating the bacillus subtilis fermentation seed solution into a fermentation tank containing a fermentation culture medium, and fermenting to obtain a bacillus subtilis fermentation solution; and mixing the bacillus subtilis fermentation solution and the urea liquid. According to the biocontrol urea prepared by the invention, antibacterial microorganisms are contained in urea particles under the condition that the fertilization habit of farmers is not changed, so that the effect of biologically preventing and treating crop diseases is achieved.

Description

technical field [0001] The invention belongs to the technical field of new fertilizers, and directly relates to a production method of biocontrol urea containing Bacillus subtilis. Background technique [0002] In the past ten years, the research on new fertilizers in my country has continued to deepen, and various new fertilizers such as controlled-release fertilizers, slow-release fertilizers, and bio-organic fertilizers have been successfully developed, which have played a positive role in promoting grain production. Due to the habit of farmers Fertilization methods have neglected the application of antiviral fertilizers, and the research on biomass antiviral fertilizers by agricultural and fertilizer research units has also been greatly affected, especially in recent years. The pests and diseases of crops are becoming more and more serious every year. It is very necessary to study a new type of biomass disease-preventing fertilizer that inhibits or kills a variety of crop ...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/60C05G5/12C12N11/04C12N11/10C12N11/14C12N1/20C12R1/125
CPCC05C9/00C05G3/00C05G3/60C05G5/12C12N11/04C12N11/10C12N11/14C12N1/20C05F11/00C05F11/08
Inventor 林迥李思华王爱云张德胜李强臧传国郭志同
Owner SHANDONG RUNYIN BIOCHEM