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Application of Sertraline in radiosensitization of glioblastoma

A glioblastoma, cell technology, applied in the field of biomedicine to achieve the effect of prolonging the survival time, enhancing the effect of radiation therapy, and prolonging the survival time

Pending Publication Date: 2022-02-01
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on any relevant application of Sertraline combined with radiotherapy in the treatment of glioblastoma

Method used

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  • Application of Sertraline in radiosensitization of glioblastoma
  • Application of Sertraline in radiosensitization of glioblastoma
  • Application of Sertraline in radiosensitization of glioblastoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening of Small Molecular Compounds Enhancing Radiosensitivity of Glioblastoma

[0029] 1. Experimental materials

[0030] Small molecule compound library (central nervous system small molecule compound library was purchased from TargetMol Company), LN229 cells, DMEM medium (containing penicillin 100U / mL, streptomycin 0.1mg / mL, 10% fetal bovine serum), Caspase-3 / 7apoptosis assay kit (purchased from Shanghai Sangong).

[0031] 2. Experimental method

[0032] 2.1 Cell Viability Detection

[0033] a. Treat LN229 cells in the logarithmic growth phase in an ultra-clean workbench, trypsinize the cells routinely, neutralize them with DMEM containing 10% fetal bovine serum after 3 minutes, wash them three times with sterile PBS, and finally collect the cells by centrifugation precipitation;

[0034] b. Resuspend the cell pellet with 1 mL of fresh medium, and count the cells with a cell counter;

[0035] c. Take an appropriate amount of the above cell suspensi...

Embodiment 2

[0056] Example 2 The therapeutic effect of Sertraline combined with radiotherapy on glioblastoma mice

[0057] 1. Experimental materials

[0058] GBM tissue samples were taken from the First Affiliated Hospital of Air Force Military Medical University (Xijing Hospital) with the informed consent of the patients and their families. Nude mice were purchased from Beijing Vital River Experimental Animal Co., Ltd., and were bred in the SPF grade animal room of the Experimental Animal Center of Air Force Military Medical University.

[0059] 2. Experimental method

[0060] 2.1 Construction of PDX model

[0061] Primary GBM samples (patients who have not received radiotherapy and chemotherapy) were directly obtained from the operating room and preliminarily processed: the surgical samples were immediately placed in a sterile tray after being separated from the body, the tumor tissue was cut open with a sterile scalpel, and 2- Three copies of tumor and paracancerous tissues were put...

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PUM

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Abstract

The invention discloses an application of a compound Sertraline in enhancing the radiotherapy sensitivity of glioblastoma. In an in-situ glioblastoma model and a patient-derived xenograft glioblastoma (PDX) model, Sertraline (15mg / kg) and radiotherapy (2Gy) are cooperated for continuous treatment for 8 days, and small animal imaging experimental results show that the tumor volume of a mouse is remarkably reduced. TUNEL staining finds that the proportion of apoptotic cells in a combined treatment group is increased, the number of Ki67 positive cells is reduced, and the expression of a glioma stem cell marker CD133 is reduced. survival curve analysis shows that the survival time of mice in a combined treatment group is remarkably prolonged. Therefore, animal experiment results disclosed by the invention show that Sertraline can effectively enhance the radiotherapy effect of glioblastoma, the survival time of mice is remarkably prolonged, and the application of Sertraline in radiotherapy sensitization of glioblastoma is fully supported.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the new role of Sertraline in synergistic radiotherapy in the treatment of glioblastoma. Background technique [0002] Glioma is the most common malignant tumor of the central nervous system, among which glioblastoma (Glioblastoma, GBM, WHO grade IV) accounts for about 54% of glioma, and has the characteristics of easy recurrence and strong invasion ability. The median survival time is only 12-15 months. Due to the malignant and invasive growth of GBM, it is often difficult to completely remove it by surgery. Radiotherapy plays an extremely important role in the treatment of GBM. Postoperative radiotherapy can effectively kill residual tumor cells and prolong the progression-free survival and overall survival of patients. For GBM that cannot be surgically removed, radiotherapy can also be used to inhibit the growth of tumor cells, improve the patient's clinical symptoms and qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/135A61P35/00A61N5/10
CPCA61K31/135A61P35/00A61N5/10
Inventor 顾锦涛张伟李萌张英起张存郝强
Owner FOURTH MILITARY MEDICAL UNIVERSITY