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A kind of tissue culture fast propagation medium and tissue culture fast propagation method of Xiaoqin silk bamboo

A technology for tissue culture, rapid propagation, and silk bamboo is applied in the directions of tissue culture, horticultural methods, botanical equipment and methods, etc., and can solve the problems of industrialization demand of bamboo seedlings, low survival rate and high cost at low cost. To achieve the effect of saving manpower, high reproduction coefficient and short production cycle

Active Publication Date: 2022-07-01
宜宾林竹产业研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the traditional Xiaoqin Sizhu breeding mainly adopts the division of female bamboo or cuttings, the survival rate is not high, and is limited by the season, there are slow speed, high cost, and it is impossible to solve the bamboo species quickly and at low cost. The problem of industrialization demand of seedlings

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  • A kind of tissue culture fast propagation medium and tissue culture fast propagation method of Xiaoqin silk bamboo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The tissue culture and fast propagation method of the violin silk bamboo of the present invention is:

[0049] (1) Selection and treatment of plant materials: select the internode with buds of the current year's branches, keep 0.5cm at the upper part of the node and 1.0cm at the lower part of the node. Rinse the cut explants once with dishwashing liquid. After rinsing, gently brush the internode stains with a fine-bristle brush, put them in a beaker, and rinse them with water for 2 hours. On the ultra-clean workbench, use 75% Sterilize with alcohol for 30 seconds, then disinfect with sodium hypochlorite 10 times solution for 8 minutes, take out the explant after it turns slightly yellow, and rinse it with sterile water 5-6 times.

[0050] (2) Induction culture: The internodes with buds of the sterilized branches of the current year were blotted with sterile absorbent paper to dry the surface water, inoculated into the induction medium, and cultured in the dark for 24 ho...

Embodiment 2

[0058] The tissue culture and fast propagation method of the violin silk bamboo of the present invention is:

[0059] (1) Selection and treatment of plant materials: select the internode with buds of the current year's branches, keep 0.5cm at the upper part of the node and 1.0cm at the lower part of the node. Rinse the cut explants once with dishwashing liquid. After rinsing, gently brush the internode stains with a fine-bristle brush, put them in a beaker, and rinse them with water for 2 hours. On the ultra-clean workbench, use 75% Sterilized with alcohol for 30 seconds, then disinfected with sodium hypochlorite 10 times solution for 8 minutes, took out the explant after it turned slightly yellow, and rinsed it with sterile water for 5 times.

[0060] (2) Induction culture: The internodes with buds of the sterilized branches of the current year were blotted with sterile absorbent paper to dry the surface water, inoculated into the induction medium, and cultured in the dark fo...

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Abstract

The invention discloses a fast propagation medium for tissue culture of bamboo silk and bamboo The components: sugar 25~35g / L and agar 3~4g / L; the proliferation medium includes MS basic medium and plant growth regulator; the proliferation medium includes the following components: 6-BA 0.03~ 3mg / L, TDZ 0.001~0.5mg / L, sugar 25~35g / L and agar 3~4g / L; the rooting medium includes MS basic medium and plant growth regulators; the root medium includes the following concentrations: Components: NAA 0.01~0.1mg / L, 6-BA 0.1~3.0mg / L, sugar 25~35g / L and agar 3~4g / L. The invention obtains the important technical effects of rapid propagation and stable rooting of the fiddlehead bamboo, and achieves the purposes of high propagation coefficient, stable technology and industrialized production.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a tissue culture rapid propagation medium and a tissue culture rapid propagation method of violet bamboo. Background technique [0002] The research on bamboo tissue culture began in the 1960s. So far, dozens of bamboo species can be obtained through tissue culture in a short period of time. However, the bamboo tissue culture technology is particularly prone to plant browning, withering, and death due to the involved adventitious bud proliferation and rooting links, which is much more difficult than other grasses tissue culture, and the medium used in each culture link. The formula is quite different due to different bamboo species, which makes the bamboo tissue culture technology not widely used in actual seedling breeding and production. [0003] Small Qin Sizhu ( Bambusa multiplex f. alphonso-karri ) is a genus of Poaceae (B ambusa Retz. corr. Schreber ) plants, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
CPCA01H4/002A01H4/008
Inventor 周国强杨海芸高会彬王勇余英郑仁红
Owner 宜宾林竹产业研究院