Preparation tool and preparation method of salt concentration gradient culture dish

Abstract

The invention provides a preparation tool and a preparation method of a salt concentration gradient culture dish. The preparation tool comprises: four ring bodies supported and fixed by a support structure. The four ring bodies are respectively a first ring body, a second ring body, a third ring body, and a fourth ring body; the axes of the four ring bodies are parallel; the bottom surfaces of the four ring bodies are flush; the first ring body is located in the second ring body and coincides with the axis of the second ring body; the diameter of the second ring body is the same as that of the third ring body; the connecting lines of the axes of the second ring body, the third ring body and the fourth ring body form an equilateral triangle. According to the preparation tool and the preparation method of the salt concentration gradient culture dish, the cutting size of an agar block containing high-concentration NaCl can be effectively unified; the positions of a salt circle, a control circle and a biological circle are accurately marked, so that the test error is greatly reduced; the structure is simple, the cost is low, and the operations are convenient.

Description

technical field [0001] The invention belongs to the technical field of animal experiment equipment, and in particular relates to a preparation tool and a preparation method of a salt concentration gradient culture dish. Background technique [0002] Due to its rapid reproduction and low cost, Caenorhabditis elegans has become one of the most widely used animal models, which plays an important role in promoting the development of biomedicine. After the various experiments and intervention methods of Caenorhabditis elegans, corresponding observation indicators are needed to test the trend and results of the experiments. Among them, the salt concentration chemotaxis test is one of the commonly used behavioral indicators because it is more objective than other sports. Behavioral detection, and the chemotaxis of nematodes can be detected, so the salt concentration chemotaxis test is a good detection index, but it takes a long time to make a salt concentration gradient test petri ...

AI Technical Summary

Problems solved by technology

[0008] In the conventional method, the circle centered on the three points ABC is basically drawn based on experience and feeling. Due to the relative error in the measurement of the ruler, and the manual circle, the consistency of the radius of the circle is not easy to control, which will lead to large errors.

And the operation time is long, increasing the experimental investment

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