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Colloidal gold immunochromatography test strip for detecting in-vivo neutralizing antibody after new crown vaccine injection and preparation method of colloidal gold immunochromatography test strip

A technology of immunochromatography test paper and colloidal gold, which is applied in the direction of immunoassay, biological test, measuring device, etc., can solve the problems that are not suitable for large-scale accompanying detection, time-consuming, complex detection operation of the kit, etc., and achieve accurate and reliable detection results , easy to operate, and the effect of less sample consumption

Pending Publication Date: 2022-02-11
无锡胜为然生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above problems in the existing technology, the present invention provides an immunochromatographic test strip and a detection method for detecting neutralizing antibodies against the novel coronavirus. The immunochromatographic test strip of the present invention can be used to determine the The content of the neutralizing antibody to the new coronavirus can produce results within 15 minutes. The test strip of the present invention can quickly diagnose the content of the neutralizing antibody with only simple operations. The result is intuitive and suitable for rapid on-site detection or household judgment
[0007] The purpose of the embodiment of the present application is to provide a novel coronavirus neutralizing antibody detection test paper to solve the technical problems of the existing kits that the detection operation is complex, time-consuming and not suitable for large-scale accompanying detection

Method used

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  • Colloidal gold immunochromatography test strip for detecting in-vivo neutralizing antibody after new crown vaccine injection and preparation method of colloidal gold immunochromatography test strip
  • Colloidal gold immunochromatography test strip for detecting in-vivo neutralizing antibody after new crown vaccine injection and preparation method of colloidal gold immunochromatography test strip

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Embodiment

[0051] An immunochromatographic test strip for detecting neutralizing antibodies against novel coronavirus such as figure 1 and figure 2 As shown, it includes a detection card and a sample diluent. The detection card includes a PVC base plate 1, a sample pad (filtered blood membrane) 2, a binding pad 3, an NC membrane 4, and absorbent paper 5. The sample pad (filtered blood membrane) film) 2, bonding pad 3, NC film 4 and water-absorbing paper 5 are overlapped and pasted on the PVC bottom plate 1 in turn, and the size of the overlapping joint is 2mm, and the size of the PVC bottom plate 1 is 80-300mm, forming The test paper board is cut into 3-4mm wide test paper strips, added with a desiccant and stored at room temperature to obtain the test card;

[0052] Among them, sample pad (blood filter membrane) 2, the size is 18*300mm, absorbent paper 5, the size is 18*300mm;

[0053] The binding pad 3 is a glass fiber pad, which is sprayed with colloidal gold labeled with the novel...

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Abstract

The invention discloses a colloidal gold immunochromatography test strip for detecting a neutralizing antibody generated in vivo after a new crown 450+150vaccine is inoculated and a preparation method of the colloidal gold immunochromatography test strip. The test strip comprises a PVC (polyvinyl chloride) bottom plate, and a sample pad (blood filtering membrane), a conjugate pad, an NC (nitrocellulose) membrane and absorbent paper are sequentially overlapped on the PVC bottom plate from left to right; the conjugate pad is coated with a recombinant novel coronavirus RBD protein marked by colloidal gold; the NC membrane is coated with human angiotensinase 2 (ACE2) as a detection line T line; and the NC membrane is coated with a chicken IgY antibody as a quality control line C line. The colloidal gold immunochromatography test strip adopts a competition method to detect a neutralizing antibody generated in the human body inoculated with a new crown vaccine, has better specificity, sensitivity and stability, is simple to operate and short in detection time, and provides a reliable choice for rapid clinical detection.

Description

technical field [0001] The invention relates to a rapid qualitative detection test strip of a blood sample and a preparation method thereof, in particular to a rapid qualitative detection test strip and its Preparation. Background technique [0002] Novel coronavirus pneumonia has swept the world, and the prevention and control of the epidemic has become a common task for governments and people of all countries. With the successive launch of COVID-19 vaccines and large-scale vaccinations across the country and even the world, the evaluation of the immune effect in individuals after vaccination has gradually attracted people's attention. [0003] The neutralizing antibodies induced in recovered patients with new coronary pneumonia and after vaccination are believed to be able to recognize the spike protein on the virus when the new coronavirus invades again and further prevent the virus from infecting cells through various mechanisms. Therefore, the detection of neutralizin...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/569G01N33/58G01N33/543G01N33/532
CPCG01N33/558G01N33/56983G01N33/587G01N33/54306G01N33/532G01N2469/20G01N2333/165
Inventor 晋榕
Owner 无锡胜为然生物科技有限公司
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