Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Genetically engineered Escherichia coli high-density fermentation medium and its fermentation process

A high-density fermentation and Escherichia coli technology, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as project stagnation, resource waste, and production that cannot achieve expected results, so as to ensure high expression and eliminate waste of resources Effect

Active Publication Date: 2022-08-02
SICHUAN BAINUOJI TECH CO LTD
View PDF12 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and development investment in biomedicine is very large, and companies that use genetically engineered E. coli to produce products often have multiple series of products expressed by genetically engineered E. coli. Repeated research and development also leads to a great waste of resources.
Some companies that transfer projects from scientific research institutes or other companies for production, due to differences in production hardware equipment, either cannot scale up production, or production fails to achieve the expected results, resulting in project stagnation, or it is difficult to achieve project transformation expectations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Genetically engineered Escherichia coli high-density fermentation medium and its fermentation process
  • Genetically engineered Escherichia coli high-density fermentation medium and its fermentation process
  • Genetically engineered Escherichia coli high-density fermentation medium and its fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] In the present embodiment, a culture medium based on the fermentation expression of the 96scFv antibody of the Escherichia coli expression system and the fermentation process adopted are provided, specifically:

[0057] Basic medium components:

[0058]

[0059] In Example 1, according to the hardware conditions of fermentation, the above-mentioned basal medium component needs to be diluted by 2 times to prepare.

[0060] Then, add the prepared medium to two-thirds of the volume of pure water, stir until there are no solid particles, and then adjust the volume, and transfer it to the fermenter.

[0061] Finally, the basal medium solution was sterilized at a sterilization temperature of 116° C. and a sterilization time of 30 minutes.

[0062] C source feed medium components:

[0063]

[0064] One part of the C source feeding medium component of this example was first added to two-thirds of the volume of pure water, stirred until there were no solid particles, and...

Embodiment 2

[0081] The present embodiment provides a culture medium based on the fermentation expression of the enzyme of the Escherichia coli expression system and the fermentation process adopted, specifically:

[0082] Basic medium components:

[0083]

[0084]

[0085] According to the hardware conditions of the fermentation, the above-mentioned basal medium components need to be concentrated by 1.5 times to prepare.

[0086] Then, the prepared basal medium components are first added to two-thirds of the volume of pure water, stirred until there are no solid particles, and then the volume is adjusted, and then transferred to the fermenter.

[0087] Finally, the basal medium solution was sterilized at a sterilization temperature of 116° C. and a sterilization time of 30 minutes.

[0088] C source feed medium components:

[0089]

[0090]

[0091] Add one part of the C source feed medium component above to two-thirds of the volume of pure water, stir until there are no sol...

Embodiment 3

[0108] The present embodiment provides a culture medium based on the fermentation expression of the sheep chlamydia disease genetic engineering subunit vaccine based on Escherichia coli expression system and the fermentation process adopted, specifically:

[0109] Basic medium components:

[0110]

[0111]

[0112] According to the hardware conditions of fermentation, one basal medium component in this example needs to be diluted 1.25 times to prepare.

[0113] Then, the prepared basal medium components are first added to two-thirds of the volume of pure water, stirred until there are no solid particles, and then the volume is adjusted, and then transferred to the fermenter.

[0114] Finally, the basal medium solution transferred into the fermenter was sterilized, and the sterilization temperature was 116° C. and the sterilization time was 30 minutes.

[0115] C source feed medium components:

[0116]

[0117] A portion of the C source feeding medium component in th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a genetically engineered Escherichia coli high-density fermentation medium and a fermentation process thereof, which are applied to the field of biotechnology. Based on the complexity of the E. coli expression system: the expression products, expression methods, expression vectors, strains and fermenters are different, and the medium and culture process used are also complex and diverse; at the same time, after the laboratory process is scaled up to production, the target product is The expression level is often very low; in view of these problems, the present invention provides a broad-spectrum medium and process: it can be applied to the high-density fermentation of most genetically engineered Escherichia coli, and can also achieve the purpose for production equipment with different conditions Mass expression of proteins to achieve maximum utilization of existing equipment.

Description

technical field [0001] The invention belongs to the biological field, and particularly relates to a high-density fermentation medium of Escherichia coli and a fermentation process thereof. Background technique [0002] Genetic engineering refers to inserting a target gene into a virus, plasmid or other carrier molecule in vitro to form a new combination of genetic material, and make it infiltrate into host cells that did not have these genes before, and can be stably inherited. With the advancement of technology, the target protein can be expressed in model organisms such as bacteria, yeast, animal cells, and plant cells. In the field of biomedicine, genetic engineering technology has been widely used to select different protein expression systems to obtain the desired target products. [0003] Among many expression systems, prokaryotic protein expression is the most common and the most economical. Among them, the E. coli expression system is widely used in the preparation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12N1/38C12R1/19
CPCC12N1/20C12N1/38
Inventor 扶星星王科刘瑞赵小欢钟彬黎宏吴福文王永胜
Owner SICHUAN BAINUOJI TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com