Preparation method and application of epsilon-polylysine derivative bio-ink
A technology of polylysine and bio-ink, applied in the direction of additive processing, etc., to achieve safe application, simple process, rapid and controllable effect
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Embodiment 1
[0020] Weigh 10 g of ε-polylysine with a molecular weight of 3500-5000, dissolve it in 100 ml deionized water at room temperature and stir until clear, and prepare a ε-polylysine solution with a concentration of 10 wt %; Aqueous acid solution and glycidyl methacrylate were mixed according to the volume ratio of 50:1, and then stirred for 6 hours under the condition of heating in a water bath at 60°C; Dialyzed for 4 days (the molecular weight cut-off of the dialysis bag is 1000), the glycidyl methacrylate modified ε-polylysine bioink was prepared by vacuum freeze-drying after dialysis, the molecular structure of ε-polylysine bioink and print the schematic as figure 1 As shown, the molecular structure of polylysine polymer grafted with methacrylic acid groups is as follows figure 2 shown.
[0021] The prepared ε-polylysine bioink powder was redissolved in deionized water to obtain a solution of ε-polylysine modified with glycidyl methacrylate at a concentration of 10 wt%; Th...
Embodiment 2
[0024] Weigh 10 g of ε-polylysine with a molecular weight of 3500-5000, dissolve it in 100 ml deionized water at room temperature and stir until clear, and prepare a ε-polylysine solution with a concentration of 10 wt %; Aqueous acid solution and glycidyl methacrylate were mixed according to the volume ratio of 50:3, and then stirred for 6 hours under the condition of heating in a water bath at 55°C; After dialysis for 4 days (the molecular weight cut-off of the dialysis bag was 1000), glycidyl methacrylate modified ε-polylysine bioink was obtained by vacuum freeze-drying after dialysis; the prepared ε-polylysine bioink The ink powder was redissolved in deionized water to obtain a solution of ε-polylysine modified by glycidyl methacrylate with a concentration of 10 wt%. Add 0.1% of ultraviolet photoinitiator LAP to this solution and Stir to dissolve it completely; at room temperature, place the glycidyl methacrylate-modified ε-polylysine solution with photoinitiator in a Nova3...
Embodiment 3
[0026] Weigh 10 g of ε-polylysine with a molecular weight of 3500-5000, dissolve it in 100 ml deionized water at room temperature and stir until clear, and prepare a ε-polylysine solution with a concentration of 10 wt %; Aqueous acid solution and glycidyl methacrylate were mixed according to the volume ratio of 10:1, and then stirred for 6 hours under the condition of heating in a water bath at 70°C; After dialysis for 4 days (the molecular weight cut-off of the dialysis bag was 1000), glycidyl methacrylate modified ε-polylysine bioink was obtained by vacuum freeze-drying after dialysis; the prepared ε-polylysine bioink The ink powder was redissolved in deionized water to obtain a solution of ε-polylysine modified by glycidyl methacrylate with a concentration of 10 wt%. Add 0.1% of ultraviolet photoinitiator LAP to this solution and Stir to dissolve it completely; at room temperature, put the glycidyl methacrylate-modified ε-polylysine solution with photoinitiator on a Nova3D ...
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