A primer-probe composition, a kit comprising the same, and a detection method thereof

A primer-probe and composition technology, which is applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems of low sensitivity, poor specificity, and low detection efficiency of kits, and achieve accurate detection. High sensitivity and sensitivity, accurate results, and good specificity

Active Publication Date: 2022-04-22
GUANGZHOU KEFEN BIOTECH CO LTD
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The second object of the present invention is to provide a joint detection kit for detecting influenza A virus, influenza B virus and respiratory syncytial virus nucleic acid, which can realize multiple inspections in one tube, and completely solve the low detection efficiency of the kit in the prior art , poor specificity and low sensitivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A primer-probe composition, a kit comprising the same, and a detection method thereof
  • A primer-probe composition, a kit comprising the same, and a detection method thereof
  • A primer-probe composition, a kit comprising the same, and a detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The primer-probe composition for detecting influenza A virus, influenza B virus and respiratory syncytial virus, and the preparation of the kit comprising it are as follows:

[0047] The primer and probe sequences in the kit are shown in Table 1 below:

[0048] Table 1 Primer, probe sequence set

[0049]

[0050] In this embodiment, the 5' end of the probe shown in SEQ ID NO:3 is marked with a VIC fluorescent group, and the 3' end is marked with a BHQ1 quenching group to ensure that influenza A viruses can be distinguished during the reaction and improve detection efficiency.

[0051] In this embodiment, the 5' end of the probe shown in SEQ ID NO: 6 is marked with a ROX fluorescent group, and the 3' end is marked with a BHQ2 quenching group to ensure that influenza B viruses can be distinguished during the reaction and improve detection efficiency.

[0052]In this embodiment, the 5' end of the probe shown in SEQ ID NO: 9 is marked with a FAM fluorescent group, and...

Embodiment 2

[0057] Methods for detecting influenza A virus, influenza B virus, and respiratory syncytial virus for non-diagnostic purposes

[0058] The detection method adopted in the present invention is q-PCR, and the PCR reaction solution, DNA polymerase, reverse transcriptase and sample nucleic acid are prepared into a nucleic acid amplification reaction mixture according to the proportion of the following reaction system for PCR reaction. The proportioning ratio of the reaction system is shown in Table 2:

[0059] Table 2 Configuration of nucleic acid amplification reaction mixture

[0060]

[0061] After the nucleic acid amplification reaction mixture is configured, the PCR reaction is carried out according to the procedure shown in Table 3:

[0062] Table 3 Fluorescent quantitative PCR reaction conditions

[0063]

[0064] Selection of fluorescence detection channels: select VIC channel to detect influenza A virus nucleic acid; select ROX channel to detect influenza B virus...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a primer probe composition, a kit containing the same and a detection method thereof, belonging to the technical field of virus detection. The specific primers and probes of cellular virus, the specific primers and probes have good specificity, adopt the test kit of the present invention, the Q-PCR method can realize the synthesis of influenza A virus, influenza B virus and respiratory tract High specificity, high sensitivity and high efficiency detection of cytomegalovirus.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and in particular relates to a primer probe composition for detecting influenza A virus, influenza B virus and respiratory syncytial virus, a kit containing the same and a detection method thereof. Background technique [0002] Respiratory tract infection (RTI) is the most common disease in humans and one of the leading causes of morbidity and mortality worldwide. The clinical manifestations caused by respiratory tract infection are mainly rhinitis, pharyngitis, laryngitis, tonsillitis and other symptoms, and severe cases can cause tracheitis, bronchitis and pneumonia, etc. However, the treatment methods, curative effects and course of the infection caused by different pathogens are also different. same. It has been proven that most of the respiratory infections are caused by respiratory viruses. Common respiratory viruses include influenza A virus, influenza B virus and respiratory sync...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/701C12Q1/686C12Q2561/113C12Q2563/107C12Q2545/114
Inventor 李娇娇
Owner GUANGZHOU KEFEN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap