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Method of extracting erythromycin from fermentation liquid by phase transition extraction

A technology of fermented liquid and erythromycin, which is applied in the field of separation engineering, can solve problems such as emulsification that plagues solvent extraction methods, and achieve the effects of easy industrial production, high extraction rate, and easy operation

Inactive Publication Date: 2004-03-10
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, the problem of emulsification has not been completely and effectively solved, and the emulsification phenomenon is still a technical problem that plagues the solvent extraction method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Mix 1000 ml of fermentation broth with erythromycin content of 6200 μg / ml and 400 ml of acetonitrile, adjust the pH value to 10.5 with ammonia water, and the temperature is 28°C. Then, add 200 grams of NaCl, stir slowly to dissolve the salt, then separate the phases of the system, the volume of the upper acetonitrile phase is 175 milliliters, the concentration of erythromycin is 34542 μg / ml, and the extraction rate is 97.5%;

[0031] Place the acetonitrile extract separated after standing in a glass still with a condenser, place the still in a constant temperature bath at 45°C, and vacuum the inside of the still with a vacuum pump until the solution boils, then condense and collect the distilled acetonitrile , when 80% (volume) of acetonitrile is evaporated, stop vacuuming, add 120 milliliters of hot water at 55 ° C, after erythromycin is separated out, filter, wash, and vacuum-dry at 45 ° C to obtain 4.835 grams of crystals, the product The biological potency is 935 un...

Embodiment 2

[0033] 1500 milliliters of fermented liquid with erythromycin content of 3200 μg / ml was equally divided into three parts, the pH value was adjusted to 10.5 with NaOH, and the temperature was 28°C. Then, 200 milliliters of acetonitrile was added to the first fermented broth, after mixing, 100 grams of NaCl was added, and after the NaCl was dissolved, 86 milliliters of acetonitrile was precipitated. The precipitated acetonitrile was added to the second fermentation broth, and 110 milliliters of acetonitrile was added. After mixing, 100 grams of NaCl was added, and 87 milliliters of acetonitrile was precipitated. The precipitated acetonitrile was added to the third fermented broth, and 110 milliliters of acetonitrile was added. After mixing, 100 grams of NaCl was added, and 88 milliliters of acetonitrile was precipitated. The concentration of erythromycin in the acetonitrile phase was 52909 μg / ml, and the total extraction rate was 97%;

[0034] The post-treatment method of the e...

Embodiment 3

[0036] Using the same method as in Example 1, but replacing NaCl with KCl, 4.423 g of crystals were obtained, and the biological potency of the product was 938 units / mg.

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PUM

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Abstract

The present invention relates to the method of extracting erythromycin from fermentation liquid by phase change extraction. Inert substance is added to the fermentation liquid to make the extractant and fermentation liquid produce phase separation, then directly extract the erythromycin from unfiltered fermentation liquid. Said method is convenient in operation, high in extraction rate and can proceed industrilization prodn.

Description

technical field [0001] The invention belongs to the technical field of separation engineering, and relates to a method for extracting erythromycin from a fermented liquid by using an extractant, in particular to a method for directly extracting erythromycin from unfiltered fermented liquid by adding a third medium to cause phase separation between the extractant and the fermented liquid. Method for extracting erythromycin from liquid. Background technique [0002] Erythromycin is an important macrolide antibiotic, its antibacterial spectrum is similar to that of penicillin G, and it is the drug of choice for the treatment of diseases caused by drug-resistant Staphylococcus aureus and hemolytic streptococci. In recent years, due to the continuous emergence of various erythromycin derivatives (such as clarithromycin, azithromycin, etc.), and people's new understanding of the pharmacological effects of erythromycin, the application field of erythromycin has been greatly expande...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/08
Inventor 乐清华宋龙林曹正芳
Owner EAST CHINA UNIV OF SCI & TECH
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