The invention belongs to the technical field of medical preparations, and discloses nanometer
chitosan ultrasonic microbubble targeting
prostate cancer cells and preparation and application thereof. A method comprises the following steps of (1) mixing
chitosan, methacryloyloxyethyl trimethyl
ammonium chloride and N-succinimidyl 3-maleimidopropionate, and heating to react, so as to obtain modified
chitosan; adding a
prostate antigen, adjusting the pH (potential of
hydrogen) value of a
system, and continuing to react, so as to obtain a complex; (2) stirring and mixing an
inert matter with low
boiling point, the complex and water, so as to obtain primary
emulsion; (3) adding a dispersant and an additive into an oil-phase
solvent, uniformly mixing, adding the primary
emulsion, stirring at high speed, adding a crosslinking agent to cure, adding a precipitating agent to precipitate, washing, centrifuging at high speed, and
drying particles, so as to obtain the ultrasonic microbubble. The nanometer chitosan ultrasonic microbubble has the advantages that the targeting property is good, the
tissue penetration ability is strong, the affinity is high, and the nanometer chitosan ultrasonic microbubble is suitable for the ultrasonic developing of the
prostate cancer cells.