Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A marker for colorectal cancer or precancerous lesions and its application

A technology for precancerous lesions and colorectal cancer, applied in the field of tumor detection, can solve problems that are not suitable for colorectal cancer screening and detection, and achieve good application prospects, high sensitivity, and avoid false positive effects

Active Publication Date: 2022-07-26
WUHAN AIMISEN LIFE TECH CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the public's acceptance of colorectal cancer blood testing is higher than that of colorectal cancer stool testing. Currently, only Septine 9 has been commercialized as a marker for blood testing. A Meta analysis (Diagnostic accuracy of methylated SEPT9 for blood -based colorectal cancer detection: a systematic review and meta-analysis. Clin TranslGastroenterol.2017; 8(1):e216.) The results showed that the sensitivity of Septine 9 for stage I colorectal cancer was 45%, and the sensitivity for polyps was 15%, making it less suitable for screening detection of colorectal cancer, so it is necessary to further search for methylation markers and their combinations suitable for blood

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A marker for colorectal cancer or precancerous lesions and its application
  • A marker for colorectal cancer or precancerous lesions and its application
  • A marker for colorectal cancer or precancerous lesions and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This embodiment provides a nucleic acid combination for colorectal cancer or precancerous lesion detection, which includes a first nucleic acid combination.

[0043] The first nucleic acid combination includes primer pair 1 (including upstream primer and downstream primer) and probe 1, and the base sequence of primer pair 1 is shown in SEQ ID NO.1-2; the base sequence of probe 1 is shown in SEQ ID NO. .5 shown.

[0044] NTMT1 upstream primer: TAGTAGGGATCGTAGTAGTTTTCGT, SEQ ID NO.1;

[0045] Downstream primer of NTMT1: ACGCCAAACCCGAAATAAAC, SEQ ID NO.2;

[0046] NTMT1 probe: TCGTCGGGTCGCGGTGAGTAA; SEQ ID NO.5.

Embodiment 2

[0048] This embodiment provides a nucleic acid combination for colorectal cancer or precancerous lesion detection, which includes a second nucleic acid combination.

[0049] The second nucleic acid combination includes primer pair 2 and probe 2. The base sequence of primer pair 2 is shown in SEQ ID NO.3-4, and the base sequence of probe 2 is shown in SEQ ID NO.6.

[0050] MAP3K14-AS1 upstream primer: GTGAGAGTATATGGGTGTGTATCGC, SEQ ID NO.3;

[0051] MAP3K14-AS1 downstream primer: CTAAAAAACAAATCGACCCGC, SEQ ID NO.4;

[0052] MAP3K14-AS1 probe: AGGGGTTGAGGTTGGGAGTCGTAGT; SEQ ID NO.6.

Embodiment 3

[0054] This embodiment provides a nucleic acid combination for colorectal cancer or precancerous lesion detection, which includes the first nucleic acid combination in Example 1 and the second nucleic acid combination in Example 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a marker for colorectal cancer or precancerous lesions and an application thereof, and relates to the technical field of tumor detection. The markers of colorectal cancer or precancerous lesions are: NTMT1 gene CpG site. Through the methylation level of the CpG site of the NTMT1 gene, early screening or auxiliary diagnosis of colorectal cancer or precancerous lesions can be achieved. The NTMT1 gene has high sensitivity and specificity in stool samples for both colorectal cancer samples or precancerous lesions. It has been verified that the detection sensitivity and specificity of NTMT1 gene methylation are much higher than those of SDC2, TFPI2, MAP3K14‑AS1 and Septine 9 genes, and NTMT1 gene is highly sensitive to stage I / II colorectal cancer and advanced adenoma in blood samples. Sensitivity and specificity for other intestinal diseases are also excellent.

Description

technical field [0001] The invention relates to the technical field of tumor detection, in particular, to a marker for colorectal cancer or precancerous lesions and an application thereof. Background technique [0002] Colorectal cancer is one of the most common malignant tumors in the world, mainly including colon cancer and rectal cancer. According to the national cancer statistics released by the National Cancer Center in 2019, the number of new cases of colorectal cancer in my country in 2015 was 376,300, and 191,000 died of colorectal cancer, ranking third in the incidence of malignant tumors and the mortality rate. fifth place. The prognosis of colorectal cancer is related to the severity of the disease at the time of diagnosis. [0003] Sporadic colorectal cancer mainly progresses in a normal-adenoma-carcinoma sequence and pattern, and early detection can significantly reduce mortality. Colonoscopy is the gold standard for colorectal cancer diagnosis, but its adhere...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 周谛晗粱嘉琳孙帆张玮
Owner WUHAN AIMISEN LIFE TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com