Micro-fluidic chip structure and method based on cell electrical characteristics and electromagnetic micro valve

Abstract

The invention discloses a micro-fluidic chip structure and method based on cell electrical characteristics and an electromagnetic micro valve, and belongs to the technical field of biomedical micro-fluidic cell sorting. The technical problems that fluid shearing force exists in existing microfluidic cell sorting, antibodies and fluorescent substances can affect the activity and function of cells, a large number of samples are needed for centrifugation, and integration with downstream processes is difficult under the condition that no manual intervention exists are solved. According to the technical scheme, the micro-fluidic chip structure comprises a solution sample inlet, a solution inlet flow channel, a solution inlet narrow channel, a detection electrode, an electromagnetic micro-valve, a micro-flow channel I, a micro-flow channel II, a waste liquid outlet and a target cell collection outlet, the control method comprises the following steps: efficiently sorting cells with target sizes by adjusting the length of a channel, the flow velocity of cell sap and the electrifying time of an electromagnet. The method has the beneficial effects that the target cells are subjected to efficient and lossless label-free sorting by utilizing the sizes and electrical characteristics of the cells.

Description

technical field [0001] The invention relates to the technical field of biomedical microfluidic cell sorting, in particular to a microfluidic chip structure and method based on cell electrical characteristics and electromagnetic microvalve. Background technique [0002] Cell sorting is of great significance in the field of biomedicine. It is usually necessary to enrich cells with the same characteristics and then further analyze them. Microfluidic technology facilitates the selective separation of cells by exploiting different properties of cells, such as size, deformability, electrical properties, magnetism, and surface markers. Many microfluidic cell sorting techniques have disadvantages, for example, fluid shear force, antibodies and fluorescent substances will affect the viability and function of cells, centrifugation requires a large amount of samples and it is difficult to integrate with downstream processes without manual intervention. [0003] How to solve the above ...

AI Technical Summary

Problems solved by technology

Many microfluidic cell sorting techniques have disadvantages, for example, fluid shear force, antibodies and fluorescent substances can affect the viability and function of cells, centrifugation requires a large amount of samples and is difficult to integrate with downstream processes without manual intervention, etc.

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