Device for long-time living body imaging of waking small animals

Abstract

The invention discloses a device and method for long-time living body imaging of a waking small animal, and the device comprises a pedestal fixed on a microscope platform or a shockproof platform, a fixed support column disposed on the pedestal, and an imaging platform disposed on the pedestal through the fixed support column, and in addition, the pedestal is also provided with a movable crawler belt. According to the device and the method, the small animal can be imaged in a waking state, imaging and signal recording in a normal physiological state are realized, and particularly, synchronous administration stimulation or electrophysiological signal recording can be carried out; the stability of an imaging area in the imaging process can be ensured, long-time stable imaging is ensured, and in addition, flexible adjustment and adaptation can be carried out according to the size of an animal.

Description

technical field [0001] The invention mainly belongs to the technical field of animal imaging, and relates to a device and method for in vivo imaging, in particular to a device and method for long-term in vivo imaging of conscious small animals. It is mainly used for small experimental animals such as rodents (such as mice, rats, guinea pigs, etc.), lagomorphs (such as rabbits, pikas, etc.), small primates (such as marmosets, etc.), especially in the awake state. In vivo, long-term imaging to study the dynamic changes of blood flow, different types of cells, and ions in small experimental animals after awakening or anesthesia. Background technique [0002] In life science research, in vivo experiments are an important supplement and verification of in vitro experiments. In in vivo research, dynamic observation of animal living tissues using a two-photon microscope is an important research method. Two-photon microscopy combines the advantages of laser confocal microscopy and ...

AI Technical Summary

Problems solved by technology

However, in fact, the real physiological state and changes of small animals can be observed and recorded more accurately only in the state of wakefulness and free movement. Due to the limitations of existing research devices, the observation of small animals in the state of wakefulness and free movement is two-photon A big problem in microscopy imaging

Common animal fixation devices are mostly used for imaging of small animals under anesthesia. Because of the existing fixation devices, small animals are generally glued to the imaging platform by dental cement, and small animals cannot move freely, and their limbs often only rest on the bottom plate. When the small animal is struggling and moving, it will have a reaction force on the bonding part, which will easily cause the bonding part to loosen and cause a large vibration, resulting in instability of the imaging focal plane. Therefore, it is usually necessary to adjust the Anesthetize the animal and keep it still

Existing methods are difficult to achieve long-term dynamic observation of small animals in an awake and free-moving state

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