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A Chemical Solvent Degrading Bacteria Pseudomonas Campylophus ph-2 and Its Application in Chemical Wastewater Treatment

A kind of Pseudomonas flexi, PH-2 technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria and other directions, to achieve the effect of low production and use cost, convenient use and good removal effect

Active Publication Date: 2022-05-03
江苏朴厚环境工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the degrading strains of MIBK. Therefore, the present invention uses MIBK as the target pollutant to domesticate the strains in high-salt and low-temperature environments to obtain a high-efficiency MIBK strain resistant to low temperature and high salt, and apply it to chemical wastewater treatment. , to provide theoretical and technical support for the application of MIBK efficient degradation microorganisms, and to provide a new microbial resource for the bioremediation of MIBK environmental pollution

Method used

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  • A Chemical Solvent Degrading Bacteria Pseudomonas Campylophus ph-2 and Its Application in Chemical Wastewater Treatment
  • A Chemical Solvent Degrading Bacteria Pseudomonas Campylophus ph-2 and Its Application in Chemical Wastewater Treatment
  • A Chemical Solvent Degrading Bacteria Pseudomonas Campylophus ph-2 and Its Application in Chemical Wastewater Treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation and screening of Pseudomonas flexus PH-2

[0031] Take 3.0ml of activated sludge obtained from the wastewater treatment tank in the production workshop of a pesticide company in Jiangsu (the company uses MIBK as a raw material solvent in the production process of some products) and add 100ml of methyl isobutyl ketone with a concentration of 200mg / L. In the inorganic salt matching medium of base isobutyl ketone, its formula is: NaCl 5.0g, (NH 4 ) 2 SO 4 1.0g, K 2 HPO 4 .7H 2 O1.96g, KH 2 PO 4 0.5g, MgSO 4 .7H 2 O 0.2g, make up to 1000 mL with deionized water, pH 7.0~7.2, sterilize at 121°C for 20 min, shake culture at 10°C, transfer to fresh inorganic salts at 3% inoculum every 10 days In the culture medium, transfer 5 times in succession.

[0032] Take 1.0ml of the enriched bacteria solution obtained above, add 9.0ml of sterile water, and make 10 -1 The rich solution, and then draw 1.0ml prepared 10 -1 The enrichment solution was added...

Embodiment 2

[0033] Embodiment 2: Pseudomonas flexus PH-2 of the present invention is tolerable to salinity

[0034] Separately configure inorganic salt liquid medium containing different NaCl concentrations (the formula is: every liter contains 200mg methyl isobutyl ketone, 1.50g K 2 HPO 4 , 0.50g KH 2 PO 4 , 0.20g MgSO 4 .7H 2 O, 1.00g NaCl, 1.00g (NH 4 ) 2 SO 4 , 20.00g agar, pH 7.0), sterilized at 121°C for 20min and cooled, then added the bacterial solution of Pseudomonas campylobacter PH-2 in Example 1 at a ratio of 1% (that is, resuspended the bacteria in 3.0ml sterile water) , at 160r / min, cultured at 30°C for 96h, respectively detected the concentration of MIBK in the inorganic salt liquid medium and the concentration of the bacterial liquid in the LB liquid medium. The result is as figure 2 It was shown that, at the concentration of 0.5~10g / L sodium chloride, Pseudomonas flexus PH-2 could effectively degrade MIBK.

Embodiment 3

[0035] Embodiment 3: the influence of temperature on the degradation effect of Pseudomonas flexus PH-2 of the present invention

[0036] Configuration methyl isobutyl ketone concentration is in the inorganic salt liquid culture medium of 200mg / L (prescription is: every liter contains 200mg methyl isobutyl ketone, 1.50g K 2 HPO 4 , 0.50g KH 2 PO 4 , 0.20g MgSO 4 .7H 2 O, 1.00g NaCl, 1.00g (NH 4 ) 2 SO 4 , 20.00g agar, pH 7.0), sterilized at 121°C for 20 minutes and cooled, then added the bacterium solution of Pseudomonas campylobacter PH-2 in Example 1 at a ratio of 1% (that is, resuspended the bacteria in 3.0ml sterile water) , set the rotation speed at 160r / min, shake and culture at 10°C, 15°C, 20°C, 25°C and 30°C for 96 hours, respectively, and measure the degradation effect by gas chromatography. The result is as image 3 It was shown that Pseudomonas camphora PH-2 can effectively degrade MIBK at 10~30℃.

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Abstract

The invention belongs to the field of chemical wastewater biological treatment, and specifically discloses a chemical solvent-degrading bacterium Pseudomonas campylobacter PH‑2 and its application in chemical wastewater treatment. Described strain Pseudomonas campylobacter, its classification name is Pseudomonas campylobacter ( Pseudomonas geniculata ), the strain name is PH‑2, and it has been deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures. The preservation date is December 6, 2021, and the preservation number is CGMCC No.24039. The Pseudomonas flexus PH‑2 described in the present invention can be applied in high-salt chemical wastewater and can withstand low temperature conditions. The bacterial agent prepared by the invention has the advantages of low production and use cost, convenient use and good removal effect, and is suitable for degrading methyl isobutyl ketone in chemical wastewater treatment throughout the country. The invention utilizes the microbial method to degrade organic pollutants, which is of great significance for improving the standard of wastewater treatment in chemical industrial parks.

Description

technical field [0001] The invention belongs to the field of biological treatment of chemical wastewater, and in particular relates to a chemical solvent-degrading bacterium Pseudomonas campylobacter PH-2 and its application in chemical wastewater treatment. Background technique [0002] In recent years, with the continuous development of the chemical industry, the amount of wastewater generated in chemical production has increased significantly, and the pollution of chemical wastewater to the environment has continued to intensify. The wastewater discharged in the chemical production process has the characteristics of complex structure, difficult to degrade, and toxicity. Therefore, its treatment process is very complicated and the capital invested in treatment is also high. Because in the process of chemical production, operations such as acid adjustment, alkali adjustment or acid-base neutralization are often involved, chemical production wastewater often has the characte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/34
CPCC12N1/20C02F3/34C02F2101/34
Inventor 邱沪生杨倩刘珍珍
Owner 江苏朴厚环境工程有限公司