Treatment method of thick blood film of blood smear for plasmodium microscopic examination

Abstract

The invention belongs to the technical field of plasmodium microscopic examination, and particularly relates to a treatment method of a thick blood film of a blood smear for plasmodium microscopic examination, which comprises the following specific steps: preparing a thick blood film and a thin blood film; performing drying; preparing a 25% ethanol (water) mixed solution while the blood membrane is dried; covering a dried thick blood film on a glass slide by using the mixed solution; enabling a 25% ethanol (water) mixed solution to act on the thick blood membrane for a certain time; drying the blood smear subjected to thick blood membrane treatment; carrying out dyeing treatment on the dried blood smear; and carrying out microscopic examination on the dyed blood smear. The treatment method can completely and effectively destroy and dissolve red blood cells in the thick blood membrane, can effectively retain plasmodium substances, nucleated white blood cells, blood platelets and the like in the red blood cells in the blood membrane, and can fix the plasmodium substances, nucleated white blood cells, blood platelets and the like in the original thick blood membrane area as much as possible, so that subsequent staining microscopic examination and accurate result judgment are ensured, and the method is suitable for large-scale popularization and application. And a more sensitive and reliable basis is provided for searching plasmodium.

Description

technical field [0001] The invention belongs to the technical field of malaria parasite microscopic examination, in particular to a method for processing blood smear thick blood film used for malaria parasite microscopic examination. Background technique [0002] Malaria is a parasitic disease caused by human Plasmodium infection. It is distributed globally, mainly in the tropics and subtropics. Malaria ranks first among the deadly parasitic diseases in the world, with about 1 million to 3 million deaths every year, most of whom are children under the age of five. After the malaria parasite invades the human body, it first develops and reproduces in the liver cells, and then invades the red blood cells, causing the red blood cells to rupture. [0003] At present, the diagnosis of malaria is mainly based on epidemiological history, typical clinical manifestations, and laboratory tests, among which the detection of Plasmodium in blood smears has the significance of diagnosis....

AI Technical Summary

Problems solved by technology

[0007] However, in clinical practice, the effects of the two methods are not satisfactory: the first method of directly adding distilled water can completely destroy red blood cells, but at the same time, all other cells on the thick blood film, including white blood cells, platelets, and malaria parasites, are basically destroyed. It will be destroyed by washing and cannot be stored on the glass slide, resulting in a thick blood film that is useless and seriously affects the subsequent staining microscope and the accuracy of the results

The second kind of Giemsa staining can not deal with the thick blood film, but the thin blood film must be fixed with methanol (CH3-OH) for 30 minutes before staining

Although this method does not need to deal with thick blood film, the staining solution needs to be diluted before use, there are many steps, and the staining time is long. During the staining process, there are still varying degrees of thick blood film shedding and incomplete destruction of red blood cells, which ultimately affects the timeliness of detection. and the result

[0008] In addition, there are domestic researchers who directly stain thick blood films with different dilutions of Wright's staining solutions. However, due to the differences in the content of individual hemoglobin and red blood cells, the effect of staining microscopy is not stable, and different degrees of thick blood film fall off or occur. incomplete hemolysis

[0009] The unsatisfactory reason for the existing thick blood film treatment method may be that the simple aqueous solution only has the effect of hypotonicity to destroy blood cells. While destroying blood cells, it also dissolves and suspends white blood cells, platelets, and malaria parasites originally fixed on the glass slide in water. And lost with water, causing all the cells in the thick blood film to lose and fall off

Images