Fermented meat product model as well as establishment method and application thereof
A fermented meat product and a technology for establishing a method are applied in the field of fermented meat product model and its establishment, which can solve problems such as difficulties in the development and optimization of fermented meat products, and achieve the effects of reducing the number of total bacteria and reducing the complex and variable influencing factors.
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Embodiment 1
[0029] The present embodiment provides a method for establishing a fermented meat product model, comprising the following steps:
[0030] S1. Raw material preparation: take fresh pork used for making fermented meat products as raw meat, take fat-free, fresh lean meat without connective tissue from the raw meat, add a total of twice the volume (1:2w / v) of 0.1 mol / L Tris-HCl buffer solution and 20mmol / L ethylenediaminetetraacetic acid solution, the meat was homogenized with a mixer under the condition of pH value 7.0. Then centrifuge at 4°C and 10000rpm for 15min, remove the supernatant containing sarcoplasmic protein, repeat the above-mentioned liquid addition, homogenization and centrifugation operation 3 times to obtain myofibrillar protein, and extract the obtained myofibrillar protein at -20 Store at ℃ for later use.
[0031] Configure simulated liquid: measure the content of free amino acids in raw meat, according to the content ratio, dissolve free amino acids in water, ...
Embodiment 2
[0034] The difference between the present embodiment and the first embodiment mainly lies in that the concentration of sodium chloride in the simulation solution of the present embodiment is 20g / L, the concentration of glucose is 8g / L, the concentration of sodium nitrite is 0.1g / L, potassium nitrate The concentration is 0.1g / L. In step S2, mix homogeneously according to the ratio of mixing 10mL of simulated liquid with 0.8g of main raw materials to form a fermented meat product model.
Embodiment 3
[0036] The difference between the present embodiment and the first embodiment mainly lies in that the concentration of sodium chloride in the simulation solution of the present embodiment is 40g / L, the concentration of glucose is 12g / L, the concentration of sodium nitrite is 0.2g / L, potassium nitrate The concentration is 0.2g / L. In step S2, according to the mixing ratio of 1.2 g of main raw materials per 10 mL of simulated liquid, the fermented meat product model is formed.
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