Molecular marker combination related to duck egg laying traits, obtaining method and application

Abstract

The invention provides a molecular marker combination related to duck egg laying traits, an obtaining method and application, and relates to the technical field of waterfowl molecular biology. According to the present invention, a No. Z sex chromosome is amplified from a female duck genome, sequencing is performed to obtain a pure molecular marker combination related to the egg laying character of the Jinding duck, the molecular combination relates to three egg laying character related gene segments, the nucleotide sequences of the three gene segments are respectively represented by SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, the molecular marker is characterized in that the 81bp position of the SEQ ID NO.1 is subjected to A81G base mutation, and the 81bp position of the SEQ ID NO.2 is subjected to A81G base mutation; the base mutation of G80A occurs at the 80bp position of the SEQ ID NO.2, and the base mutation of A127G occurs at the 127bp position of the SEQ ID NO.3. The molecular marker combination can provide a rapid, simple, convenient and low-price gene analysis method for early breeding of the egg laying performance of the Jinding ducks, is beneficial to screening out Jinding duck individuals with relatively good egg laying performance, and guides early molecular marker-assisted breeding of the egg laying performance of the Jinding ducks.

Description

technical field [0001] The invention relates to the technical field of waterfowl molecular biology, in particular to a combination of molecular markers related to duck laying traits, an acquisition method and an application thereof. Background technique [0002] Retinoic acid-related orphan nuclear receptor ROR is one of the subfamilies of the nuclear receptor family, consisting of three subtypes: RORa, RORb, and RORc. ROR plays an important role in the development, immunity, biological rhythm, and cell metabolism of mammals. Played a key role, among which the regulation of biological rhythm is mainly played by RORb. Studies have shown that RORb - / - Male mice showed a delay in fertility [Andre et al., 1998], Wang (2015) et al. found differential expression of RORb in the ovary transcriptome sequencing of blue and white pigeons, Chen (2021) et al. Differentially expressed genes of RORb were screened in follicles, so it was speculated that RORb may be related to egg productio...

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Problems solved by technology

[0003] Arylsulfatase B (ARSB) is a lysosomal enzyme, also known as N-acetylgalactosamine-4-sulfatase, and in humans, multiple studies have shown that deficiency of this enzymatic activity leads to Accumulation of degraded substrates and disorder of lysosomal storage, that is, Maroteaux-Lamy disease (mucopolysaccharidosis type VI), has not yet been reported in poultry

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