Biological liquid treatment system and operation method thereof

Abstract

The invention discloses a biological liquid treatment system and an operation method thereof. The system consists of a disposable biological liquid treatment device and matched biological liquid treatment equipment, wherein the biological liquid treatment device further comprises an air bag; the top end of the piston type separation cup is provided with a liquid channel and an air channel which are communicated with the outside inside the cup body; the connecting pipeline consists of a sample pipeline, a waste liquid pipeline, a product pipeline, an air pipeline and a pressure monitoring pipeline; one end of the air pipeline is connected with the air bag, and the other end of the air pipeline is connected with an exhaust port of the air channel; the biological liquid treatment equipment further comprises an air valve and an ultrasonic air detector. When the separation cup is applied, the liquid channel and the gas channel can be controlled to be communicated or closed with the separation cup body according to needs, so that the concentration efficiency of biological liquid is improved; the existing operation of detaching and inverting the centrifugal cup is avoided, and the work of manually pushing the piston is saved; the position change of the piston can be accurately monitored, and the liquid amount in the separation cup can be accurately metered.

Description

technical field [0001] The invention belongs to the technical field of biological liquid separation, and in particular relates to a biological liquid processing system and an operating method thereof. Background technique [0002] In the field of biological cell processing, a processing system based on the density gradient principle is often used to separate biological fluids, such as separating mononuclear cells in single blood collection, concentrating and washing the cultured cell fluid. The piston-type separation cup is shaped like a syringe, including a cup body and a movable piston, and the piston is driven by a power device to suck or discharge liquid. Due to its flexible and variable treatment volume, the treatment system with piston separation cup is widely used in the field of biological liquid treatment. [0003] Current biological fluid handling systems include disposable biological fluid handling devices as well as biological fluid handling equipment. The curr...

AI Technical Summary

Problems solved by technology

[0013] 1. The current separation cup has only one channel, and both suction and discharge go through this channel. In the first cycle, there must be a special exhaust process (ie suction→extrusion→resuction), and the efficiency is low

[0014] 2. When the amount of sample liquid is greater than the volume of the separation cup (that is, when multiple processing cycles are required), it is necessary to use the waste liquid to recover the unseparated sample liquid in the pipeline into the sample bag, which has certain influence on the design of the consumable pipeline. Certain restrictions; and if there is an error in the metering system, it is easy to discharge the "unseparated sample liquid" into the waste liquid bag

However, the optical device used to monitor the position of the piston has high precision, and the sensors used are controlled by foreign manufacturers, so it is easy to get stuck

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