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Sample preservation solution for culturing organoids

A technology for preserving fluid and organoids, which is applied in the field of sample preservation fluid for culturing organoids, which can solve problems such as organoid culture failure, and achieve the effect of improving the success rate and improving the activity.

Pending Publication Date: 2022-04-26
宋伟 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, organoid culture will be adopted by more and more research institutions and researchers, but in the specific organoid culture process, due to the activity of biological samples, many organoid cultures fail.

Method used

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  • Sample preservation solution for culturing organoids
  • Sample preservation solution for culturing organoids
  • Sample preservation solution for culturing organoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the preparation of preservation solution: optimization formula 1

[0051] Preservation solution components: Advance DMEM / F12, N2(0.5×), B27(1×), Gluta MAX Supplement(1×), HEPES(1×), Y-27632(100μM), bovine serum albumin(5%) , holotransferrin (1mg / L), insulin (1mg / L), L-glutathione (0.2mg / L), sodium selenite (0.01mg / L), ethanolamine hydrochloride (2mg / L ), N-acetylcysteine ​​(5mM), EGF (50ng / ml), FGF10 (200ng / ml).

Embodiment 2

[0052] Embodiment 2, preparation of preservation solution: optimization formula 2

[0053] Preservation solution components: Advance DMEM / F12, N2(1×), B27(1×), Gluta MAX Supplement(1×), HEPES(1×), Y-27632(150μM), bovine serum albumin(5%) , holotransferrin (5mg / L), insulin (2mg / L), N-acetylcysteine ​​(10mM), sodium pyruvate (15mM), FGF 2 (10ng / ml), FGF 7 (25ng / ml ).

Embodiment 3

[0054] Embodiment 3, the preparation of preservation solution: optimization formula 3

[0055] Preservation solution components: Advance DMEM / F12, N2(0.1×), B27(0.1×), Gluta MAX Supplement(1×), HEPES(1×), Y-27632(200μM), bovine serum albumin(10%) , sodium selenite (0.05mg / L), ethanolamine hydrochloride (10mg / L), β-mercaptoethanol (250μM), FGF10 (250ng / ml), Normocin TM (2x), dimethylsulfoxide (DMSO) 1% (v / v).

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Abstract

The invention discloses a sample preservation solution for culturing organoids. By optimizing the proportion of the components of the preserving fluid, the activity of animal biological samples can be improved, and a material with excellent biological activity is provided for subsequent culture of organoids, so that the culture success rate is increased. And a foundation is laid for providing efficient materials for organoid culture.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a sample preservation solution for culturing organoids, which can significantly improve the success rate of organoid culture after preservation. Background technique [0002] Apoptosis occurs after the biological sample loses the support of the original in vivo environment, and the cell activity decreases rapidly, which affects the subsequent detection and cell culture expansion that require high cell activity. Most of the existing biological sample activity preservation solutions are aimed at the preservation of intracellular nucleic acids and proteins, and there are few more efficient preservation solutions for preserving the overall activity of cells in larger tissue samples. [0003] In clinical scientific research, it is often necessary to obtain patient samples for more detection and research involving cell activity, especially for the current relatively cutting-edge organoid re...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/071C12N5/09
CPCA01N1/0226C12N5/0693C12N5/0679C12N2500/25C12N2500/38C12N2500/46C12N2500/20C12N2500/30C12N2501/11C12N2501/115C12N2501/117C12N2501/119
Inventor 宋伟柏卫华
Owner 宋伟