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Micro-fluidic chip for sorting sperms

A microfluidic chip and sorting technology, which is applied in the field of microfluidic chips for sperm sorting, can solve the problems such as the selection efficiency and the accuracy rate to be improved, and achieve sorting efficiency and high-throughput sperm sorting and sorting. Efficient and easy-to-operate effect

Pending Publication Date: 2022-04-29
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as sperm selection is concerned, the selection efficiency and accuracy of current microfluidic chips still need to be improved

Method used

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  • Micro-fluidic chip for sorting sperms
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] see figure 1 The technical solution provided by the present invention is to implement a microfluidic chip for sperm sorting based on sperm flow taxis, with a supporting glass as the substrate 1, the length of which is 20mm, the width is 10mm, and the height is 2mm.

[0029] The material of the upper flexible material 3 is PDMS, and its length is 20mm, width is 10mm, and height is 3mm.

[0030] The main flow channel 4 is located on the central axis of the upper layer of flexible material 3, and has a length of 10 mm, a width of 1 mm, and a height of 100 μm.

[0031] The collection structure 2 is located inside the main channel 4 and is a channel in the shape of a funnel. The bell mouth of the funnel is connected to the wall of the main channel 4, and its material is the same as that of the upper flexible material 3, which is PDMS. The length of the collection structure 2 is 1 mm, the height is 100 μm, the thick bottom is 20 μm, the angle between the bell mouth and the m...

Embodiment 2

[0040] see figure 1 The technical solution provided by the present invention is to implement a microfluidic chip for sperm sorting based on sperm flow taxis, with a supporting PEI as a substrate 1, with a length of 100 mm, a width of 90 mm, and a height of 30 mm.

[0041] The material of the upper flexible material 3 is hydrogel, and its length is 100mm, width is 90mm, and height is 50mm.

[0042] The main channel 4 is located on the central axis of the upper layer of flexible material 3, and has a length of 90 mm, a width of 6 mm, and a height of 1.5 mm.

[0043] The collection structure 2 is located inside the main channel 4 and is a channel in the shape of a funnel. The bell mouth of the funnel is connected to the wall of the main channel 4, and its material is the same as that of the upper flexible material 3, which is hydrogel. The collecting structure 2 has a length of 6 mm, a height of 1.5 mm, a thick bottom of 300 μm, an angle between the bell mouth and the main chann...

Embodiment 3

[0052] see figure 1 The technical solution provided by the present invention is to implement a microfluidic chip for sperm sorting based on sperm flow taxis, using PS with a supporting function as the substrate 1, with a length of 30 mm, a width of 20 mm, and a height of 4 mm.

[0053] The upper layer of flexible material 3 is made of PDMS, with a length of 30 mm, a width of 20 mm, and a height of 6 mm.

[0054] The main channel 4 is located on the central axis of the upper layer of flexible material 3, and has a length of 20 mm, a width of 2 mm, and a height of 200 μm.

[0055] The collection structure 2 is located inside the main channel 4 and is a channel in the shape of a funnel. The bell mouth of the funnel is connected to the wall of the main channel 4, and its material is the same as that of the upper flexible material 3, which is PDMS. The collecting structure 2 has a length of 1 mm, a height of 200 μm, a thick bottom of 30 μm, an angle between the bell mouth and the ...

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Abstract

The invention discloses a micro-fluidic chip for sorting sperms, which is used for sorting high-quality sperms from seminal fluid by utilizing the taxis characteristic of the sperms. According to the micro-fluidic chip, a substrate with a supporting function is used as a lower layer; the upper layer is made of a flexible material engraved with channels and a collecting structure, and the channels comprise a vertical sample introduction channel and a horizontal branch sample introduction channel which are distributed on the two sides of the main flow channel, a vertical buffer solution inlet channel, a vertical buffer solution outlet channel, and a vertical sample outlet channel and a horizontal branch sample outlet channel which are located on the two sides of the main flow channel. The flexible material on the upper layer and the substrate on the lower layer are tightly attached together. The sperm sorting chip has the beneficial effects that the sperm sorting chip has the advantages of simplicity and convenience in preparation, low cost, easiness in operation, high sorting efficiency and the like; besides, high-flux sperm sorting can be realized, so that the efficiency of sperm sorting is greatly improved, and the method has a wide application prospect in the field of assisted reproduction.

Description

technical field [0001] The invention relates to the field of sperm screening in assisted reproduction, in particular to a microfluidic chip for sperm sorting. Background technique [0002] Reproduction, as an inevitable way for human beings to reproduce offspring, is crucial to the inheritance of human civilization, and she is an indispensable part of human social activities. However, with the development of society, changes in the external environment and living habits, infertility has become more and more common around the world. According to the data survey results of 33 research centers in 25 countries by the World Health Organization (WHO), the incidence of infertility in developed countries is about 5% to 8%, and this incidence is developing Some areas in China are even as high as 30%. Men account for more than half of infertility patients. According to data published by the WHO, as many as one in five healthy men aged 18 to 25 have abnormal sperm counts, with only ...

Claims

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Application Information

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IPC IPC(8): C12M1/00B01L3/00
CPCC12M23/16B01L3/5027B01L3/502761
Inventor 刘兵孙斐刘倩
Owner NANTONG UNIVERSITY
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