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Lycium barbarum polysaccharide extract with function of resisting lung cancer A549 cell proliferation

A technology of Lycium barbarum polysaccharides and extracts, which is applied in the field of functional macromolecular screening, can solve the problems of lack of specificity in radiotherapy and chemotherapy, toxic and side effects of patients, etc., and achieve good inhibitory effect, easy purification, and strong activity

Active Publication Date: 2022-05-06
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003]The treatment of tumors including lung cancer has always been a hot spot in clinical medical research and new drug development. However, due to the lack of specificity, traditional radiotherapy and chemotherapy often give patients Bring relatively large toxic and side effects, and traditional Chinese medicines including single herbal medicines and traditional Chinese medicine compound prescriptions have emerged in this regard, and quickly play an important role, with broad development prospects

Method used

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  • Lycium barbarum polysaccharide extract with function of resisting lung cancer A549 cell proliferation
  • Lycium barbarum polysaccharide extract with function of resisting lung cancer A549 cell proliferation
  • Lycium barbarum polysaccharide extract with function of resisting lung cancer A549 cell proliferation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Preparation of Lycium barbarum polysaccharide crude extract

[0019] l, preparation of extract I:

[0020] Take 50g of wolfberry, add 15 times the amount of water, decoct at 80°C for 4 hours, collect the extract by filtration, and concentrate to obtain extract I.

[0021] 2. Preparation of polysaccharide crude extract:

[0022] Add ethanol to the extract I to a final concentration of 40%, 50%, 60%, and 70% respectively. After standing still, centrifuge at 10,000 rpm for 10 minutes. The obtained precipitates are named 40%p, 50%p, and 60%p respectively. , 70%p; the crude extracts of the above four polysaccharides were prepared into a solution with a concentration of 100 μg / mL.

[0023] Take different lung cancer cells in the logarithmic growth phase, digest and resuspend, and adjust the cell concentration to 6x10 3 cells / mL, inoculated into 96-well culture plate, 0.2 mL per well. After continuing to cultivate for 24 hours, each 6 wells was an experimenta...

Embodiment 2

[0025] Example 2: Inhibitory effect of purified components of Lycium barbarum polysaccharide crude extract on A549 cell proliferation

[0026] 60% alcohol-precipitated polysaccharide 60%p (named crude polysaccharide p in this example) was eluted with deionized water and NaCl solutions with concentrations of 0.2% and 0.5%, respectively, to obtain eluents, which were freeze-dried respectively Three different polysaccharide fractions were obtained, namely water-washed polysaccharide P-1, 0.2% NaCl eluent P-2, and 0.5% NaCl eluent P-3.

[0027] According to the steps of Example 1, the inhibitory effect of human lung cancer A549 cells (the used human lung cancer A549 cells were donated by the Translational Medicine Center of Gansu Cancer Hospital) was analyzed. Proliferation was significantly inhibited.

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Abstract

The invention provides a lycium barbarum polysaccharide extract with a function of resisting lung cancer A549 cell proliferation. The lycium barbarum polysaccharide extract is obtained by adding water into lycium barbarum, decocting, filtering, adding ethanol into filtrate until the final concentration is 60%, standing, precipitating and centrifuging. The lycium barbarum polysaccharide extract and the washed polysaccharide component provided by the invention are used for inhibiting proliferation of lung cancer A549 cells. The lycium barbarum polysaccharide obtained through a water extraction and alcohol precipitation method has the advantages of being easy to obtain and purify and high in activity, and cell experiments find that the lycium barbarum polysaccharide has a good inhibition effect on lung cancer A549 cells.

Description

technical field [0001] The invention belongs to the technical field of functional macromolecule screening and application, and in particular relates to a wolfberry polysaccharide extract with anti-proliferation function of lung cancer A549 cells. Background technique [0002] Lung cancer (lung cancer) originates in the bronchial mucosa and alveolar epithelial cells. It is one of the common malignant tumors in the world today, and has ranked first among malignant tumors in recent years. Especially with the aging of the population, the proportion of urban population increases year by year, the rapid development of urban industrial production, the further deterioration of environmental pollution, smoking and other reasons, its incidence is on the rise in our country. [0003] The treatment of tumors including lung cancer has always been a hot spot in clinical medical research and new drug development. However, due to the lack of specificity, traditional radiotherapy and chemoth...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P11/00A61P35/00
CPCC08B37/0003A61K31/715A61P35/00A61P11/00
Inventor 邸多隆马文锦刘建飞
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI