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SNP (Single Nucleotide Polymorphism) molecular marker linked with peanut bacterial wilt resistant major QTL (Quantitative Trait Loci) and application thereof

A molecular marker, anti bacterial wilt technology, applied in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., to achieve the effect of improving breeding efficiency

Active Publication Date: 2022-05-06
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the mapping of QTLs for resistance to bacterial wilt in peanuts, and only a stable major QTL and its molecular markers have been discovered on the B02 chromosome

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker linked with peanut bacterial wilt resistant major QTL (Quantitative Trait Loci) and application thereof
  • SNP (Single Nucleotide Polymorphism) molecular marker linked with peanut bacterial wilt resistant major QTL (Quantitative Trait Loci) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This example provides the development process of the SNP molecular marker BWRB03-R linked to the main QTL site qBWRB03 for resistance to bacterial wilt in peanut, as follows.

[0037] Test materials: A peanut bacterial wilt-susceptible variety Zhonghua 10 was used as the female parent, and a peanut bacterial wilt-resistant germplasm ICG12625 was used as the male parent to obtain a recombinant inbred line (RIL )group.

[0038] Phenotype identification: In 2019 and 2020, the bacterial wilt resistance of 140 strains of Zhonghua 10, ICG12625 and RIL populations was identified in the Hongan bacterial wilt disease nursery of the Institute of Oil Crops, Chinese Academy of Agricultural Sciences. A completely randomized block experimental design was adopted, with 3 repetitions. Each time, each material was planted in a row of 20 plants, with a row spacing of 30 cm and a plant spacing of 10 cm. Adopt standard field management practices. After all the seedlings emerged, the tot...

Embodiment 2

[0045] This example provides the 131 RILs of ICG12625 and Zhonghua 10 that obtained survival rate data for both environments in Example 1 by using the SNP molecular marker BWRB03-R linked to the major bacterial wilt resistance locus qBWRB03 to test. Specific steps are as follows.

[0046] Using genomic DNA as a template and the sequences shown in SEQ ID NO.2-3 as primers, the SNP molecular marker BWRB03-R was amplified with the KAPA2G Fast MultiplexMix kit. The PCR conditions were: 95°C pre-denaturation for 3 minutes; 95°C denaturation for 15 s, 55°C renaturation for 30 s, 72°C extension for 30 s, a total of 30 cycles; finally 72°C extension for 5 min, 4°C incubation. After the amplification product was added with a sequencing adapter, the Illumina HiSeq platform was used for Paired-end 150bp (PE150) sequencing, and the sequencing sequence was compared to the reference sequence to detect the base of the SNP site. If the RIL is consistent with the base of the SNP site detecte...

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Abstract

The invention relates to the technical field of molecular markers, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker linked with a peanut bacterial wilt resistant major QTL (Quantitative Trait Loci) and application of the SNP molecular marker. According to the SNP molecular marker BWRB03-R provided by the invention, an SNP polymorphic site is located at the 201st site of a sequence as shown in SEQ ID NO.1, and the polymorphism is G / A; specifically, in the SNP molecular marker BWRB03-R, the genotype of the polymorphic site is A, the corresponding peanut phenotype has relatively strong bacterial wilt resistance, the genotype of the polymorphic site is G, and the corresponding peanut phenotype has relatively weak bacterial wilt resistance. The SNP molecular marker provided by the invention can assist in selecting a peanut material resistant to bacterial wilt, is beneficial to promoting the breeding of a peanut variety resistant to bacterial wilt, and improves the breeding efficiency.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a SNP molecular marker linked to the main QTL site qBWRB03 for peanut bacterial wilt resistance and its application. Background technique [0002] Peanut (Arachis hypogea L.) is one of the important oil crops. Bacterial wilt caused by Rastonia solanacearum is the most important bacterial disease harmful to peanut production. Ralstonia solanaceare invades the roots of plants from the soil, expands upward along the vascular bundles, and multiplies in large numbers, eventually causing the plant vessels to lose their ability to transport water and die quickly. In peanut production, the fatality rate of plants in general diseased areas is 10-30%, and the fatality rate of plants in severely diseased areas can reach more than 80%. Therefore, effective prevention and control of bacterial wilt is one of the major demands for the development of peanut industry. [0003] Breedi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2535/122
Inventor 廖伯寿罗怀勇雷永晏立英姜慧芳陈玉宁刘念淮东欣周小静黄莉康彦平陈伟刚王欣王志慧
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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