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Preparation method of kit for detecting sulfydryl metabolites in urine

A technology for detecting kits and metabolites, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reactions of materials, etc., can solve the problems of low accuracy, complicated operation, cross-infection, etc. Achieve high accuracy and specificity

Pending Publication Date: 2022-05-06
梅宗泉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are more complicated to operate, the accuracy is low, the subject has certain trauma, and there is cross-infection at the same time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: (Prepare the reagent solution according to the volume ratio of each tube of the kit to make 500 kits)

[0014] 1. The solution of the test reagent is distilled water, and 100ml of distilled water is prepared;

[0015] 2. The solution of the contrast reagent is a mercuric chloride solution, and 100ml of a 0.2mol / l mercuric chloride solution is prepared;

[0016] 3. The chromogenic agent is a mixture of phosphotungstic acid reagent solution and buffer solution at a volume ratio of 1:1, 2000ml.

[0017] 1. Preparation of phosphotungstic acid reagent solution, weigh 61g of sodium tungstate, dissolve in 320ml of distilled water, add 35ml of 85% phosphoric acid, reflux and boil for 2 hours, then cool to room temperature, add 15g of iron sulfate, fully dissolve, add distilled water Dilute to 1000ml. Finally, add 35g of lithium sulfate and 50ml of 0.5% seaweed gel, shake well, and store in a refrigerator at 2°C-8°C for later use.

[0018] 2. The pH of the buffer s...

Embodiment 2

[0020] Example 2: (Prepare the reagent solution according to the volume ratio of each tube of the kit to make 500 kits)

[0021] 1. The solution of the test reagent is distilled water, and 100ml of distilled water is prepared;

[0022] 3. The chromogenic agent is a mixture of phosphotungstic acid reagent solution and buffer solution at a volume ratio of 1:1, 2000ml.

[0023] 1. Preparation of reagent solutions such as phosphotungstic acid, weigh 65g of sodium tungstate, dissolve in 350ml of distilled water, add 40ml of 85% phosphoric acid, reflux and boil for 2 hours, then cool to room temperature, add 20g of iron sulfate, fully dissolve, add Distilled water to make up to 1000ml. Finally, add 35g of lithium sulfate and 55ml of 0.5% seaweed gel, shake well, and store in a refrigerator at 2°C-8°C for later use.

[0024] 2. The pH of the buffer solution is 5.7. According to the requirements of the pH value, prepare 1000ml of phosphate buffer solution (sodium dihydrogen phosphat...

Embodiment 3

[0026] Example 3: (Prepare the reagent solution according to the volume ratio of each tube of the kit to make 1000 kits)

[0027] 1. The solution of the test reagent is distilled water, and 200ml of distilled water is prepared;

[0028] 2. The solution of the contrast reagent is mercuric chloride solution, and 200ml of mercuric sulfate solution of 0.5mol / l is prepared;

[0029] 3. The chromogenic agent is a mixture of phosphotungstic acid reagent solution and buffer solution at a volume ratio of 1:1, 4000ml.

[0030] 1. Preparation of phosphotungstic acid reagent solution, weigh 80g of sodium tungstate, dissolve in 700ml of distilled water, add 80ml of 85% phosphoric acid, reflux and boil for 2 hours, then cool to room temperature, add 18g of iron sulfate, fully dissolve, add distilled water Dilute to 2000ml. Finally, add 65g of lithium sulfate and 65ml of 0.5% seaweed gel, shake well, and store in a refrigerator at 2°C-8°C for later use.

[0031] 2. The pH of the buffer so...

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Abstract

The invention relates to a preparation method of a detection kit for sulfydryl metabolites in urine, the detection kit comprises a control reagent, a test reagent and color developing test paper, a solution of the test reagent is distilled water, a solution of the control reagent is a mercuric chloride solution or a mercuric sulfate solution, and a solution of the color developing test paper is distilled water. The chromogenic test paper is prepared by uniformly mixing a phosphotungstic acid reagent solution and a buffer solution according to a volume ratio of 1: 1, soaking qualitative low-speed filter paper in a chromogenic agent solution, taking out the filter paper, drying the filter paper in a box for 1 hour, and packaging the filter paper with a plastic bag. The detection kit disclosed by the invention has the characteristics of high accuracy, good specificity and the like, 215 diagnosed cervical cancer patients are detected, 188 patients are positive, and the positive coincidence rate is 87.4%; the reagent is used for screening 385 cases of general population, 7 cases are positive, the positive rate is 1.8%, and the specificity is 98.2%; meanwhile, 27 positive cases are obtained by using similar reagents on the market to detect the same crowd, the positive rate is 7%, and the specificity is 93%.

Description

technical field [0001] The invention relates to a preparation method of a detection kit, in particular to a preparation method of a detection kit for sulfhydryl metabolites in urine. Background technique [0002] A significant increase in the level of sulfhydryl metabolites in female urine can indicate the occurrence of cervical cancer. Currently common cervical cancer screening methods include HPV (human papillomavirus) detection, TCT (liquid-based thin layer cytology), Pap smear detection and pathological diagnosis. These methods are more complicated to operate, and the accuracy is on the low side, and the subject has a certain trauma, and there is cross-infection at the same time. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of a detection kit for sulfhydryl metabolites in urine, which is simple in operation, high in accuracy, and capable of avoiding wounds and cross-infections in subjects. [0004] The pre...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 李润慈蔡云
Owner 梅宗泉
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