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Automatic processing method and device for carrying out proteomic analysis on FFPE sample

A proteomics and processing method technology, applied in the field of biotechnology automation, can solve the problems of difficult extraction of paraffin section samples, long experiment cycle, low extraction efficiency, etc., and achieve the effect of improving sample processing efficiency

Pending Publication Date: 2022-05-10
天津诺禾致源科技有限公司
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  • Application Information

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Problems solved by technology

[0004] The present invention aims to provide an automatic processing method and device for proteomics analysis of FFPE samples, so as to solve the technical problems of difficult extraction of paraffin section samples, low extraction efficiency and long experimental period in the prior art

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  • Automatic processing method and device for carrying out proteomic analysis on FFPE sample
  • Automatic processing method and device for carrying out proteomic analysis on FFPE sample
  • Automatic processing method and device for carrying out proteomic analysis on FFPE sample

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Experimental program
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Embodiment 1

[0041] Sample: Paraffin section of human tissue

[0042] Reagent:

[0043] Dewaxing reagent: Xylene

[0044] Hydration reagents: 100% ethanol, 75% ethanol, 50% ethanol and PBS

[0045] Protein lysate contains: 4% SDS, 100mM Tris, 10mM TCEP, 40mM CAA and 100mM NaCl.

[0046] acetone

[0047] The DB reconstitution solution contains: 8M urea and 100mM TEAB.

[0048] Relevant experimental parameter settings: add dewaxing reagent, shake and mix for 30s, incubate at room temperature for 10 minutes, centrifuge at 6000g for 2 minutes, remove supernatant, repeat the operation once; add hydration reagents in sequence: 100% ethanol, 100% ethanol, 75% ethanol, 50 % ethanol, PBS, shake and mix for 30s after each addition of hydration reagent, incubate at room temperature for 5 minutes, centrifuge at 6000g for 2 minutes, remove supernatant; add protein lysate, shake and mix for 30 seconds, incubate at 95°C and 1000rpm for 60 minutes; cool to room temperature Then add four times the vol...

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Abstract

The invention discloses an automatic processing method and device for carrying out proteomic analysis on an FFPE sample. The automatic processing method comprises the following steps: S1, a first moving module places a sample tube in which an FFPE sample is placed at a first preset position; s2, identifying the sample tube by an identification unit to obtain identification information of the sample tube; s3, selecting a target production mode based on the identification information, the target production mode being a mode for processing the FFPE sample; and S4, processing the FFPE sample based on the target production mode to obtain a processing result. By applying the technical scheme, the purpose of automatically processing the FFPE sample can be achieved through the automatic processing method, the technical effect of improving the sample processing efficiency is achieved, and then the technical problems that in the related technology, the proteomics pretreatment of the FFPE sample needs manual participation and is low in efficiency are solved.

Description

technical field [0001] The invention relates to the field of biotechnology automation, in particular to an automatic processing method and device for proteomics analysis of FFPE samples. Background technique [0002] In biopsy testing, formalin-fixed paraffin-embedded (FFPE) has been used to preserve the histological and morphological structures of tissues for histopathological diagnosis of diseases. FFPE tissues are highly stable and can be stored at room temperature for a long time. As a result, a large number of normal and pathological FFPE samples containing information relevant to diagnosis, treatment, and outcome are generated worldwide. Thus, FFPE tissue banks are now a potentially valuable resource for retrospective studies of biomarker discovery and validation for cancer and other important diseases. Combining high-throughput proteomic analysis technology with FFPE will reveal a large amount of disease-related information, which will play an important role in dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/36G01N1/38C12P21/06C07K1/30C07K1/14G01N33/68
CPCG01N1/36G01N1/38C12P21/06C07K1/30C07K1/14G01N33/68
Inventor 余文祥张宝刘巧赵晓雯张伟王忍李艳萍
Owner 天津诺禾致源科技有限公司