Supercharge Your Innovation With Domain-Expert AI Agents!

Method for preparing glycated albumin calibrator and quality control product

A technology for glycosylated albumin and quality control products, applied in the field of biomedicine, can solve the problems of cumbersome processing steps, difficulty in taking into account cost and process convenience, and many instruments and equipment.

Pending Publication Date: 2022-05-13
URIT MEDICAL ELECTRONICS CO LTD +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing glycosylated albumin calibrator and quality control product, aiming at solving the difficulty of taking into account the cost and process convenience of the traditional preparation method of glycosylated albumin calibrator and quality control product in the prior art, Some methods have cumbersome processing steps, long production cycle, more instruments and equipment, and technical problems that are difficult to carry out preparation work under general laboratory conditions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing glycated albumin calibrator and quality control product
  • Method for preparing glycated albumin calibrator and quality control product
  • Method for preparing glycated albumin calibrator and quality control product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Specifically, embodiment 1, a method for preparing glycated albumin calibrator and quality control product, comprises the following steps:

[0062] S1: Porcine serum processing and porcine serum albumin raw material preparation

[0063] S1.1: Take pig serum, use a high-speed centrifuge at 6000rpm-8000rpm, centrifuge for 20min-30min, discard the precipitate, and keep the supernatant;

[0064] S1.2: Take the centrifuged pig serum raw material, measure the volume accurately, slowly add solid ammonium sulfate, stir while adding, so that its saturation is 50%, after the addition, continue stirring for 15min-30min, until the ammonium sulfate is completely dissolved ;

[0065] S1.3: Use a high-speed centrifuge to centrifuge at 6000rpm-8000rpm for 20min-30min, discard the precipitate, and keep the supernatant;

[0066] S1.4: Use GE polysulfone membrane tangential flow ultrafiltration column to ultrafilter the supernatant after dialysis and centrifugation, control the concentr...

Embodiment 2

[0089] Specifically, embodiment 2, a method for preparing glycated albumin calibrator and quality control product, comprises the following steps:

[0090] S1: Porcine serum processing and porcine serum albumin raw material preparation

[0091] S1.1: Take pig serum, use a high-speed centrifuge at 7000rpm-9000rpm, centrifuge for 20min-30min, discard the precipitate, and keep the supernatant;

[0092] S1.2: Take the centrifuged pig serum raw material, measure the volume accurately, slowly add solid ammonium sulfate, and stir while adding, so that its saturation is 60%. After adding, continue to stir for 15-30 minutes until the ammonium sulfate is completely dissolved ;

[0093] S1.3: Use a high-speed centrifuge to centrifuge at 7000rpm-9000rpm for 20min-30min, discard the precipitate, and keep the supernatant;

[0094] S1.4: Use Fresenius hollow fiber ultrafiltration column to ultrafilter the supernatant after dialysis and centrifugation, control the concentration of albumin af...

Embodiment 3

[0117] Specifically, Embodiment 3, a method for preparing glycated albumin calibrator and quality control product, comprising the following steps:

[0118] S1: Porcine Serum Processing and Porcine Blood Albumin Raw Material Preparation

[0119] S1.1: Take pig serum, centrifuge at 8000rpm~10000rpm with a high-speed centrifuge for 20min~30min, discard the precipitate, and keep the supernatant;

[0120] S1.2: Take the raw porcine serum after centrifugation, measure the volume accurately, slowly add solid ammonium sulfate, and stir while adding, so that its saturation is 60%, after adding, continue stirring for 15min-30min, until the ammonium sulfate is completely dissolved ;

[0121] S1.3: Centrifuge at 8000rpm~10000rpm for 20min~30min with a high-speed centrifuge, discard the precipitate, and keep the supernatant;

[0122] S1.4: Use Fresenius hollow fiber ultrafiltration column to ultrafiltration and dialysis the supernatant after centrifugation, and control the albumin concen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biological medicines, and particularly discloses a method for preparing a glycated albumin calibrator and a glycated albumin quality control product, which comprises the following steps: treating porcine serum and preparing an albumin raw material; preparing a glycated albumin low-value quality control product; preparing high-value raw materials of a glycated albumin calibration product and a quality control product; adding a freeze-drying protective agent into the prepared glycated albumin calibrator and the quality control product, uniformly stirring, and sub-packaging and freeze-drying; and main performance index tests of accuracy and stability are carried out on the prepared product by using a corresponding experimental scheme. According to the method disclosed by the invention, the problems of high biological safety risk, low yield, poor stability, high cost and long production period of the original technical process are solved by utilizing a method of combining salting-out and ultrafiltration.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for preparing a glycated albumin calibrator and a quality control product. Background technique [0002] Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia caused by defects in insulin secretion caused by genetic and environmental factors. Long-term hyperglycemia can lead to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. Diabetic patients have typical symptoms of polyuria, polydipsia, polyphagia, and weight loss, and late complications can be fatal. In recent years, diabetes has an obvious upward trend in my country, and it has become the third most serious chronic disease that threatens health after tumor and cardiovascular disease. Diagnosis, treatment and monitoring of diabetes are of great significance to maintaining people's health. [0003] Glycated albumi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/765C07K1/34C07K1/30C07K1/14G01N33/68
CPCC07K14/765G01N33/6893G01N2333/765G01N2800/042
Inventor 张成林叶志杰覃肖珍黄艳妮
Owner URIT MEDICAL ELECTRONICS CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com