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Application of Ctrp15 in preparation of products for promoting angiogenesis and improving ischemia-related cardiac functions

A product, the technology of myocardial ischemia, applied in the field of medicine, can solve problems such as poor myocardial repair

Active Publication Date: 2022-05-27
BEIJING INST OF HEART LUNG & BLOOD VESSEL DISEASES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main treatment method for patients with myocardial infarction is to restore blood supply through reperfusion therapy. However, there are still a large number of patients with poor myocardial repair and complications such as heart failure due to untimely surgery or ischemia-reperfusion injury.

Method used

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  • Application of Ctrp15 in preparation of products for promoting angiogenesis and improving ischemia-related cardiac functions
  • Application of Ctrp15 in preparation of products for promoting angiogenesis and improving ischemia-related cardiac functions
  • Application of Ctrp15 in preparation of products for promoting angiogenesis and improving ischemia-related cardiac functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Exercise-induced Ctrp15 protein secreted by skeletal muscle

[0034] 1. Exercise promotes functional recovery after cardiac ischemia and hypoxia injury

[0035] WT wild mice were selected, and the swimming experiment and acute myocardial infarction model were used to calculate the survival rate, cardiac function changes and pathological level of the mice after myocardial infarction to evaluate the changes of cardiac remodeling, and to clarify the effect of exercise on the protection of the heart on the posterior side of myocardial infarction. The degree of branch angiogenesis and cardiac function repair.

[0036] (1) Construction of the motion model

[0037] Swimming simulation exercise: start swimming for 10 minutes per day and gradually increase by 10 minutes per day, until after 50 minutes per day, exercise 6 days a week for 4 weeks to obtain exercise model mice.

[0038] (2) Construction of an animal model of myocardial infarction

[0039] The mouse mo...

Embodiment 2

[0119] Example 2. Application of Ctrp15 protein in the treatment or alleviation of cardiac injury

[0120] The supplementary protein overexpressing Ctrp15 is used to detect whether it can effectively alleviate cardiac injury: On the premise of successful model construction, a myocardial infarction model was established by using WT wild mice, and the supplemental protein overexpressing Ctrp15 was used to determine whether the exercise prescription based on Ctrp15 was confirmed by cardiac ultrasound and pathological detection. Therapeutic effect of promoting collateral angiogenesis on cardiac function repair after myocardial infarction.

[0121] Injured heart mice (MI) were treated with the protein Ctrp15 in vivo:

[0122] (1) In the same way as the heart injury animal model of Example 1, a mouse model of myocardial infarction (non-exercise group) was obtained;

[0123] (2) Preparation of protein Ctrp15:

[0124] The protein Ctrp15 (company: AVISCERA BIOSCIENCE; product number...

Embodiment 3

[0139] Example 3. Knockout of CTRP15 significantly aggravated heart damage after myocardial infarction

[0140] In order to clarify the protective effect of Ctrp15 on cardiac function, gene CTRP15 KO mice were constructed; WT and CTRP15 KO mice were simultaneously ligated the anterior descending coronary artery to establish a myocardial infarction model. Effects of cardiac injury after myocardial infarction.

[0141] (1) Construction of CTRP15 knockout mice

[0142] Compared with wild-type C57BL / 6 mice, CTRP15 knockout mice (named CTRP15-KO mice) only have the base C at position 5122 of the CTRP15 gene (sequence 2) deleted in the genome, and other genes remain unchanged.

[0143] The above-mentioned CTRP15 gene knockout mice can be prepared by using site-directed mutagenesis or other existing techniques, and the present invention takes the following as an example:

[0144] CTRP15 systemic knockout mice were constructed from Cyagen Biosciences.

[0145] The identified gene s...

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Abstract

The invention discloses an application of Ctrp15 in preparation of a product for promoting angiogenesis and improving ischemia-related cardiac functions. The invention provides an application of Ctrp15 protein or a coding gene thereof or a substance taking the Ctrp15 protein as an active component in at least one of the following applications. 1) preparing a product for treating heart injury; 2) preparing a product for relieving heart injury; and 3) preparing a product for improving the cardiac function repair capability after heart injury. The invention finds that the Ctrp15 protein secreted by the skeletal muscle induced by movement can improve the collateral angiogenesis after myocardial infarction and finally promote the repair of cardiac functions by researching whether the Ctrp15 regulates the collateral angiogenesis or not in the movement process and researching a molecular mechanism.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to the application of Ctrp15 in the preparation of a product for promoting angiogenesis and improving ischemia-related cardiac function. Background technique [0002] Myocardial infarction (MI) is the death of myocardial ischemia and hypoxia caused by acute coronary occlusion, which is the main cause of heart failure and death. Among them, about 1 / 3 of the patients were found to have old myocardial infarction and sufficient collateral circulation at the time of admission for treatment; and clinical studies have found that the long-term prognosis of patients is significantly related to the degree of new collateral circulation. The incidence of heart failure (3.1% vs 11.6%) and mortality (7.1% vs 15.4%) after 60 months of reperfusion therapy in well-established patients were significantly lower than those in inadequately established patients. At present, the main treatment method for patients...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P9/00A61P9/10C12Q1/6883G01N33/68
CPCA61K38/1709A61P9/10A61P9/00C12Q1/6883G01N33/6893G01N2800/324C12Q2600/158
Inventor 杜杰乔博康李玉琳王媛贾立昕张聪聪王春筱
Owner BEIJING INST OF HEART LUNG & BLOOD VESSEL DISEASES
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