Agarose microspheres with high pressure resistance three-stage structure and preparation method of agarose microspheres
An agarose microsphere, tertiary structure technology, applied in separation methods, chemical instruments and methods, other chemical processes, etc., can solve the problems of long IgG retention time and low pressure resistance, and achieve strong rigidity and fast penetration rate. Effect
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Embodiment 1
[0029] A kind of agarose microsphere with high pressure three-stage structure and preparation method thereof, comprising the following steps:
[0030](1) Preparation of modified agarose: 4.8g of agarose added to 120g of water, put into a magnetic stirrer to boil 100 ° C, boil for 1min, remove the cooling for 3min, then put in the magnetic stirrer, boil for 1min, then remove the bottle, then cool for 3min, and then put in the magnetic stirrer, boil for 1min, to get 4% aqueous agarose solution. Take 4% aqueous agarose solution 10mL, add it to the flask at 50 °C while hot, and then add 5g of 5g of methacrylic acid glycidyl ether to the flask, add preheated 50 μL 45% NaOH solution, mechanically stirred at a speed of 200 rpm, stirred the reaction for 8h, to be cooled and layered, remove the upper transparent solution, to obtain a solid methacrylic acid glycidyl ether modified agarose, the pore size of the modified agarose microsphere after polymerization is about 100 nm.
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Embodiment 2
[0034] A kind of agarose microsphere with high pressure three-stage structure and preparation method thereof, comprising the following steps:
[0035](1) Preparation of modified agarose: 4.8g of agarose added to 120g of water, put into a magnetic stirrer to boil 100 ° C, boil for 1min, remove the cooling for 3min, then put in the magnetic stirrer, boil for 1min, then remove the bottle, then cool for 3min, and then put in the magnetic stirrer, boil for 1min, to get 4% aqueous agarose solution. Take 4% aqueous agarose solution 10mL, add it to the flask at 50 °C while hot, and then add 5g of 5g of vinyl glycidyl ether to the flask, add preheated 50 μL 45% NaOH solution, mechanically stirred at a speed of 200 rpm, stirred the reaction for 8h, wait for the reaction to be cooled and layered, remove the upper layer of transparent solution, to obtain a solid vinyl glycidyl ether modified agarose, the pore size of the modified agarose microspheres after polymerization is about 100 nm.
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Embodiment 3
[0039] A kind of agarose microsphere with high pressure three-stage structure and preparation method thereof, comprising the following steps:
[0040](1) Preparation of modified agarose: 4.8g of agarose added to 120g of water, put into a magnetic stirrer to boil 100 ° C, boil for 1min, remove the cooling for 3min, then put in the magnetic stirrer, boil for 1min, then remove the bottle, then cool for 3min, and then put in the magnetic stirrer, boil for 1min, to get 4% aqueous agarose solution. Take 4% aqueous agarose solution 10mL, add it to the flask at 50 °C while hot, and then add 5g of 5g of vinyl glycidyl ether to the flask, add preheated 50 μL 45% NaOH solution, mechanically stirred at a speed of 200 rpm, stirred the reaction for 8h, wait for the reaction to be cooled and layered, remove the upper layer of transparent solution, to obtain a solid vinyl glycidyl ether modified agarose, the pore size of the modified agarose microspheres after polymerization is about 100 nm.
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