Embryo peripheral fluid detection system

Abstract

The invention discloses an embryo peripheral liquid detection system which comprises a shell, and an objective table for placing an embryo small dish, a probe mechanism for detecting embryo peripheral liquid in the embryo small dish and an imaging device for collecting image information of the embryo small dish are arranged in the shell. An analysis and control device for analyzing embryo peripheral liquid and controlling the probe mechanism to work is arranged outside the shell, the input end of the analysis and control device is connected with the output end of the imaging device, and the output end of the analysis and control device is connected with the controlled end of the probe mechanism; the method for detecting the peripheral liquid of the embryo by using the system comprises the following steps: culturing the embryo; embryo observation; assembling a probe; and detecting the embryo peripheral fluid. According to the method, embryo culture can be achieved, metabolites or secretory proteins in peripheral liquid can be detected in real time, the limitation of embryo quality evaluation of traditional embryo morphology is solved, and the reliability of embryo quality evaluation is improved.

Description

technical field [0001] The invention relates to the technical field of embryo detection, in particular to an embryo peripheral fluid detection system. Background technique [0002] Embryo quality is one of the key factors for successful pregnancy. The developmental potential of embryos, that is, the evaluation of embryo quality, directly affects the screening of embryo transfer and pregnancy outcome. [0003] At present, the evaluation of embryo quality is mainly based on the traditional morphological method, that is, according to the law of embryo fertilization and division, the embryo morphology is observed at specific time points, and the embryo quality is evaluated and graded according to the morphology standard, and the selection level is the highest. embryos for transfer. However, this kind of morphological detection has great limitations, relying on the subjective judgment of observers, and there is no accurate and objective evaluation standard; and the so-called mor...

AI Technical Summary

Problems solved by technology

[0004] The limitations of morphological evaluation of embryo quality are: 1) The significance of morphological indicators is often limited to the surface layer, and there is no direct causal relationship with the quality of embryos; 2) The morphological description is affected by the subjective judgment of the evaluator. Insufficient professional experience may lead to unreliable evaluation; 3) The division and definition standards of morphological indicators are not perfect enough, such as the abnormal morphological changes of embryos at different developmental stages do not have sufficient resolution, and a large number of statistical studies are still needed

The existing technical solutions include genetic evaluation of embryos, using preimplantation genetic diagnosis (PGD) technology, extracting blastomeres or trophoblast cells of embryos for genetic analysis to find abnormalities in embryonic chromosomes and genes, This test is more meaningful for patients with familial genetic disorders; however, the effect of mechanical sampling on subsequent development of the embryo is unknown

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