Biliary atresia evaluation system, method for detecting MMP-7, kit and application

A technology of MMP-7 and detection kits, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of false negative results, pain of children, trauma, etc., to reduce the negative rate, avoid missed diagnosis and misdiagnosis, The effect of improving the prognosis

Pending Publication Date: 2022-05-31
WUXI DIAGNOSTICS LAB SHANGHAI CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liver biopsy may have false-negative results, and is limited by age, requiring multiple punctures to confirm the diagnosis, causing great pain to children
[0007] In the case where the above-mentioned conventional examinations cannot make a diagnosis, the final diagnosis of biliary atresia still depends on intraoperative exploration and postoperative pathology. The trauma of these examinations is relatively large, and nearly 20% of children are not found to be biliary atresia after surgery.

Method used

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  • Biliary atresia evaluation system, method for detecting MMP-7, kit and application
  • Biliary atresia evaluation system, method for detecting MMP-7, kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] This kit is limited to the detection of human serum samples.

[0085] Serum samples can be stored for 7 days under the conditions of 2°C to 8°C; and 6 months under the conditions of -15°C-25°C.

[0086] Try to avoid the repeated freezing and thawing of the sample, and the number of freezing and thawing is no more than 6 times.

[0098] 3.11 coverslips;

[0100] 4.1 Equilibrate the serum sample and the kit to room temperature (20°C to 25°C);

[0101] 4.2 Preparation of 1X washing solution: the washing solution (20X) was diluted 20 times with purified water, for subsequent use.

[0102] 4.3 Pre-diluting the serum sample: dilute the sample 20 times with the sample diluent, such as 190 μL sample dilution

[0103] 4.4 Add 100 μL of diluted samples to each well of the sample wells; add 100 μL of calibrator and 100 μL of each of the quality control products to each well of the calibration quality control wells

[0105] Automatic plate washing: wash 4 times with 1X washing solution,...

Embodiment 2

[0121] The dried blood slice quantitative collection device adopts the dried blood slice quantitative collection device disclosed by CN212513820U. Quantification of dried blood tablets

[0123] Test sample: dried blood sheet. Collection site: If blood is collected from the fingertip, take the inner side of the ring finger of the right hand; if blood is collected from the heel, take the small

[0135] Take out the 96-well plate, discard the liquid in the well, add 300 μL washing solution to each well after patting dry, and discard the washing after shaking for 30 seconds

[0148] Add 100 μL of enzyme-labeled secondary antibody, and incubate for one hour at 37°C;

[0150] Add 100 μL of substrate TMB, shake and mix, and incubate at 37° C. for ten minutes after sealing;

[0151] Add stop solution 50 μL;

Embodiment 3

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PUM

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Abstract

The invention provides a biliary atresia evaluation system, a method for detecting MMP-7, a kit and application. The biliary atresia assessment system is a biliary atresia risk assessment system based on MMP-7 and bile acid spectra, and the biliary atresia assessment system takes the concentration of MMP-7 in a subject sample and the concentration of bile acid in the subject sample as input and takes a risk assessment result as output. By adopting the technical scheme, the infantile biliary atresia can be conveniently, efficiently and accurately evaluated, screened and / or diagnosed, the diagnosis accuracy of the biliary atresia is remarkably improved, and non-invasive accurate diagnosis is realized.

Description

Biliary atresia assessment system, method, kit and application for detecting MMP‑7 technical field The invention belongs to in vitro diagnostic technology field, be specifically related to biliary atresia assessment system, the method for detecting MMP-7, Kits and Applications. Background technique Biliary atresia is a severe hepatobiliary surgical disease in children, characterized by obstruction of extrahepatic bile ducts, which in turn leads to intrahepatic bile. Accumulation, liver damage, progressive liver fibrosis, if not diagnosed and treated in time, the child will suffer from liver failure within 1-2 years of age And death. According to statistics, liver transplants caused by biliary atresia account for the majority of liver transplants for infants under one year old. 60% or so. And the highest incidence in Asia. Portojejunostomy (Kasai portoenterostomy, KP), namely Kasai surgery, is the first choice for biliary atresia. treatment plan. Clinical studi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N33/543G01N33/573G01N33/58G01N33/68G16H10/60G16H50/20
CPCG01N33/573G01N33/6893G01N33/581G01N33/54306G01N30/02G01N30/72G16H50/20G16H10/60G01N2333/96494
Inventor 方焯郑珊邓嘉乐杨大可
Owner WUXI DIAGNOSTICS LAB SHANGHAI CO LTD
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