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Method for removing o-dichlorobenzene, m-dichlorobenzene and p-dichlorobenzene in water by mucor circinelloides and application of method

A kind of technology of Mucor curcumae and o-dichlorobenzene, applied in the field of microorganisms, can solve the problems of undisclosed removal of dichlorobenzene, biodegradation, unproven tolerance and resistance, etc., and achieves the effect of strong degradation effect

Active Publication Date: 2022-06-03
YANCHENG INST OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent does not disclose that the strain can be applied to remove dichlorobenzene, and its biodegradation, tolerance and resistance have not been proven

Method used

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  • Method for removing o-dichlorobenzene, m-dichlorobenzene and p-dichlorobenzene in water by mucor circinelloides and application of method
  • Method for removing o-dichlorobenzene, m-dichlorobenzene and p-dichlorobenzene in water by mucor circinelloides and application of method
  • Method for removing o-dichlorobenzene, m-dichlorobenzene and p-dichlorobenzene in water by mucor circinelloides and application of method

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Embodiment 1

[0047] Embodiment 1: the application of a kind of Mucor curcumae FNZJ3-2-2 in removing ortho-, meta-, and para-mixed dichlorobenzene in eutrophic waste water,

[0048] 1. Bacterial preparation

[0049] Take 1000 mL of the logarithmic phase of the LB culture medium of Mucor cribrosa, centrifuge at 4 °C for 10 min at 13000 r / min, add sterile water and repeat the centrifugation 3 times, and keep the wet cells at a constant weight as viable cells, and store at 4 °C for later use .

[0050] 2. Single factor test of biodegradation of FNZJ3-2-2 viable cells

[0051] Take an appropriate amount of the viable cells of Mucor sclerotiorum FNZJ3-2-2 to make the bacterial density in the system 2~10×10 8 pcs / mL, added to the eutrophic wastewater containing three kinds of mixed dichlorobenzene, o, m and p, at pH6.0~pH8.0, degradation temperature of 28~36℃, three kinds of mixed dichlorobenzene, o, m and p The concentration is 25-125mg / L (1:1:1 of the three dichlorobenzenes between the two g...

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Abstract

The invention discloses a method for removing o-dichlorobenzene, m-dichlorobenzene and p-dichlorobenzene in water through mucor circinelloides and application of the method. The preservation name of the mucor circinelloides FNZJ3-2-2 is Mucor circinelloides FNZJ3-2-2, the mucor circinelloides FNZJ3-2-2 is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.9684, and the preservation date is September 19, 2014; the eutrophic wastewater is an inorganic salt liquid culture medium containing o-dichlorobenzene, m-dichlorobenzene and p-dichlorobenzene; the composition of the inorganic salt liquid culture medium is K2HPO43H2O; naH2PO4 (sodium hydrogen phosphate); the mucor circinelloides FNZJ3-2-2 has the advantages that the mucor circinelloides FNZJ3-2-2 viable bacteria are proved by single-factor experiments, and accordingly the mucor circinelloides FNZJ3-2-2 viable bacteria have high degradation effects on o-dichlorobenzene, m-dichlorobenzene and p-dichlorobenzene in the eutrophic wastewater;

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for removing ortho, meta and para mixed dichlorobenzene in water by Mucor curcumae and application thereof. Background technique [0002] Compared with the traditional chemical technology and physical technology for removing organic pollutants, biodegradation is more economically and environmentally feasible, and has become a promising environment-friendly and energy-saving sewage (waste) water and waste gas treatment technology. Studies have shown that a variety of microorganisms have good biodegradation effects on a variety of single organic pollutants or combined organic pollutants. Microorganisms have strong adaptability and mutagenicity. All organic pollutants can be mutated to form corresponding degrading microorganisms. The use of microorganisms to degrade organic pollutants has very high application prospects for remediation. [0003] Persiste...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/14C12R1/785C02F101/36
CPCC02F3/34C12N1/14C02F2101/36Y02A50/20
Inventor 顾乃鹏孙国凤赵雨慧仇树梅李朝霞孙飞丁成于云江马卫星
Owner YANCHENG INST OF TECH