Automatic focusing microscopic imaging system based on optical fiber bundle and focusing method

Abstract

The invention provides an automatic focusing microscopic imaging system and focusing method based on an optical fiber bundle, and the device comprises a detection light beam generated by a light source module, and the detection light beam is reflected by a light splitting module to generate a first light beam; reflected light generated by projecting the first light beam to the sample through the objective lens is returned to the light splitting module through the objective lens and reflected to generate a second light beam; the second light beam generates a light spot signal through an optical fiber module and is transmitted to a photoelectric detector for photoelectric conversion to generate an electric signal; the control module generates a focusing instruction based on the electric signal to control and adjust the focal plane position of the objective lens; imaging signal light generated by the sample under the illumination light source is returned to the light splitting module through the objective lens with the focal plane position adjusted to be transmitted to generate a third light beam, the third light beam is imaged through the imaging module, and an image of the sample is obtained. According to the automatic focusing microscopic imaging system based on the optical fiber bundle and the focusing method provided by the invention, the internal structure can be simplified, the accuracy of defocusing amount and defocusing direction calculation can be improved, and the cost can be reduced.

Description

technical field [0001] The invention relates to the field of optical technology, in particular to an automatic focusing microscopic imaging system and focusing method based on an optical fiber bundle. Background technique [0002] With the rapid development of current biological detection, medical diagnosis and other fields, higher requirements are put forward for the response speed, imaging resolution and precision of modern optical microscopes. The manual focusing method of traditional microscopes relies on human judgment, requires a lot of repetitive operations, is time-consuming and labor-intensive, and has low reliability. For this reason, real-time autofocus technology that can quickly compensate for microscopic imaging focus drift becomes the key to stable imaging. [0003] At present, autofocus methods are mainly divided into active focus and passive focus. Passive focusing uses the image sharpness evaluation function to determine the position of the focal plane ac...

AI Technical Summary

Problems solved by technology

[0005] The present invention provides an automatic focusing microscopic imaging system and focusing method based on optical fiber bundles, which are used to solve the defects of high cost and complex structure caused by relying on quadrant detectors to collect electrical signals and calculate defocus in the prior art, and to realize The optical fiber module and ordinary photodetectors formed by multiple single fibers in different arrangements capture the electrical signals at various positions in the detection area to calculate the defocus amount, improve the calculation accuracy and save costs

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