Application of bacterial load as marker in predicting recurrence and metastasis risk of nasopharynx cancer

A technology for nasopharyngeal carcinoma and bacteria, which is applied in the application field of bacterial load as a marker in predicting the risk of recurrence and metastasis of nasopharyngeal carcinoma, and can solve the problems of unknown clinical significance and judgment criteria of tumor flora

Pending Publication Date: 2022-07-08
SUN YAT SEN UNIV CANCER CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the nasopharynx is an important niche of the upper respiratory tract microbiota, the clinical significance and criteria of the tumor microbiota in nasopharyngeal carcinoma remain unknown

Method used

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  • Application of bacterial load as marker in predicting recurrence and metastasis risk of nasopharynx cancer
  • Application of bacterial load as marker in predicting recurrence and metastasis risk of nasopharynx cancer
  • Application of bacterial load as marker in predicting recurrence and metastasis risk of nasopharynx cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0070] Example 2 Discovery of critical value of bacterial load for high and low risk recurrence and metastasis risk

[0071] The inventors included patient samples from 241 previously untreated, non-metastatic, locally advanced nasopharyngeal carcinoma patients who had undergone radical radiotherapy. 93% of the patients (223 cases) received cisplatin-based concurrent chemotherapy. After 88.2 months of follow-up, 61 of them developed recurrence or metastasis during the follow-up period.

[0072] The bacterial load of 241 nasopharyngeal carcinoma patient tissues was obtained according to the above method, and the maximum χ was generated by the Mantel-Cox test using X-tile software. 2 Value scores were used to classify patients into "low" and "high" risk groups. First, X-tile divides the data into two partitions, and performs the correlation test between the data each time it is divided. figure 2 The color of A represents the strength of the association of each partition, ran...

Embodiment 3

[0074] Example 3 Predictive performance of critical value of bacterial load

[0075] The applicant further conducted a univariate Cox regression analysis and found that the clinical outcomes of nasopharyngeal cancer patients in the training group, the Guangzhou internal validation group and the Zhejiang external validation group were significantly correlated with the bacterial load in the tumor, which indicated that the bacterial load in the tumor was significantly related to the nasal cavity. The prognosis of pharyngeal cancer is closely linked ( Figure 4 ). Further by multivariate Cox regression analysis, the applicant found that intratumoral bacteria was the main factor for disease-free survival of nasopharyngeal carcinoma (training group: HR 2.61, 95%CI 1.54-4.43; p<0.001; internal validation group: HR 3.65, 95%CI2. 23-5.97; p<0.001; external validation group: HR 2.21, 95%CI 1.42-3.44; p<0.001), no distant metastasis (training group: HR 2.98, 95%CI 1.47-6.02; p=0.002; in...

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Abstract

The invention discloses an application of a bacterial load as a marker in predicting recurrence and metastasis risks of nasopharyngeal carcinoma, and discloses that the bacterial load can be used for predicting high-low risk recurrence and metastasis risks and prognosis of the nasopharyngeal carcinoma for the first time, and the prediction of lifetime reveals and verifies that the bacterial load in a tumor is a valuable biomarker in the nasopharyngeal carcinoma. And the critical value of the bacterial load in the nasopharynx cancer tumor is determined to be 206.4 copies / microliter, and the prediction efficiency on non-disease survival is superior to that of tumor TNM staging. Meanwhile, quantitative analysis of nasopharyngeal carcinoma tissue bacterial load based on qPCR provides an economic, simple and convenient solution for clinic, and also provides a new insight for accurate individualized treatment of nasopharyngeal carcinoma patients.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to the application of bacterial load as a marker in predicting the risk of recurrence and metastasis of nasopharyngeal carcinoma. Background technique [0002] Nasopharyngeal carcinoma originates from nasopharyngeal epithelial cells and is an aggressive head and neck malignancy. Because the symptoms of nasopharyngeal carcinoma are not obvious in the early stage, more than 70% of the patients are in the middle and late stages when they first visit a doctor. The main reasons for treatment failure are recurrence and distant metastasis in the lung and liver. At present, TNM staging (T is primary tumor, N is lymph node, and M is distant metastasis) is widely used to guide the prognosis and treatment strategy of NPC patients. However, local recurrence and / or distant metastasis occurs in approximately 15% to 30% of patients of the same stage who receive similar treatment re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6886C12N15/11G16H50/30
CPCC12Q1/689C12Q1/6886G16H50/30C12Q2600/118
Inventor 柳娜马骏乔涵谭细容黎映琴何庆梅黄圣燕龚莎叶明亮
Owner SUN YAT SEN UNIV CANCER CENT
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