Pathological sample preparation and desoxyribonucleic acid quantitative staining instrument and use method thereof

A deoxyribonucleic acid and staining instrument technology, applied in the field of medical machinery, can solve the problems of troublesome operation and increase the cost of labor, and achieve the effect of avoiding pollution and good air tightness

Pending Publication Date: 2022-07-08
MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quantitative staining of deoxyribonucleic acid is mostly done manually, or different machines are used for film preparation and staining. Strictly speaking, the whole process is equivalent to semi-automation, and the operation is cumbersome, which increases the cost of manpower.

Method used

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  • Pathological sample preparation and desoxyribonucleic acid quantitative staining instrument and use method thereof
  • Pathological sample preparation and desoxyribonucleic acid quantitative staining instrument and use method thereof
  • Pathological sample preparation and desoxyribonucleic acid quantitative staining instrument and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Mode 1: Make 1 slide + Pasteur staining (gradient centrifugation + automatic transfer + natural sedimentation + Pasteur staining)

[0044] Pre-treatment: specimen, slide number, shake the specimen; add 4ml of separation solution to the centrifuge tube, add 8ml of specimen; centrifuge at 1080r for 2min, discard 8ml of the upper separation solution; centrifuge again for 2000r, 10min, discard the remaining liquid; add 1ml of buffer liquid, and shake to mix.

[0045] Loading the tablet: Go on the machine, set the machine working temperature to 25°C, add 800ul buffer, and mix for N times; transfer 800ul of the suspension to the sedimentation chamber with the robotic arm, settle for 10 minutes, and aspirate and discard the waste liquid from the sedimentation chamber; wash 3 with 1ml of cleaning solution For the last time, let the washing solution stand for 1 minute, then aspirate and discard the washing solution; rinse 3 times with 1 ml buffer, aspirate and discard the buffer...

Embodiment 2

[0049] Mode 2: Making 2 slides + DNA staining (gradient centrifugation + automatic transfer + natural sedimentation + DNA staining)

[0050] Pre-treatment: specimen, slide number, shake the specimen; add 4ml of separation solution to the centrifuge tube, add 8ml of specimen; centrifuge at 1080r for 2min, discard 8ml of the upper separation solution; centrifuge again for 2000r, 10min, discard the remaining liquid; add 1ml of buffer liquid, and shake to mix.

[0051]Loading the tablet: Go to the machine, set the machine working temperature to 35°C, add 800ul buffer, and mix for N times; transfer 800ul of the suspension to sedimentation chamber 1 and sedimentation chamber 2, and settle for 10 minutes; suction and discard sedimentation chamber 1, sedimentation Warehouse 2 waste solution; add 1ml buffer, aspirate and discard; wash 3 times with 1ml washing solution, and let the last washing solution stand for 1 minute. Settling bin 2 sucks and discards the cleaning solution, settli...

Embodiment 3

[0056] Mode 3: Make 2 slices separately (gradient centrifugation + automatic transfer + natural sedimentation)

[0057] Pretreatment: Specimen, slide number, shake the specimen; add 4ml of separation solution to the centrifuge tube, add 8ml of specimen; centrifuge at 1080r for 2min, discard 8ml of the supernatant separation solution; centrifuge again for 2000r, 10min, discard the remaining liquid; add 1ml of buffer liquid, and shake to mix.

[0058] Loading the tablet: put on the machine, add 800ul buffer, mix N times; transfer 800ul suspension to sedimentation chamber 1, sedimentation chamber 2, and settle for 10 minutes; suction and discard the waste liquid of sedimentation chamber 1 and sedimentation chamber 2; add 1 ml Buffer, aspirated and discarded; 1 ml of washing solution was washed 3 times, the last washing solution was allowed to stand for 1 minute, and the washing solution was aspirated and discarded.

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Abstract

The invention discloses a pathological sample preparation and desoxyribonucleic acid quantitative staining instrument which is characterized by mainly comprising four modules, namely a sample preparation module, a desoxyribonucleic acid quantitative staining module, a temperature control module and a system control module, as well as a waste liquid collection device, a waste gas collection device and a waste solid collection device. The invention also discloses a method for operating the stainer. According to the technical scheme, the integrated preparation and staining of the pathological sample can be realized, and the stainer is safe, environment-friendly, convenient to use, complete in process coverage and elastic and flexible in program selection. The requirements of standardization and automation of a pathology department laboratory are met.

Description

technical field [0001] The invention relates to the technical field of medical machinery, in particular to a pathological sample preparation and deoxyribonucleic acid quantitative staining instrument. Background technique [0002] With the increasing demand for robots and automated processing in the development of modern industries, special medical and life science industries in all walks of life have higher and higher requirements for test automation. At present, the quantitative staining of DNA is mostly manual operation, or different machines are used for production and staining. Strictly speaking, the whole process is equivalent to semi-automation, which is more troublesome to operate and increases the cost of labor. Deoxyribonucleic acid quantitative staining is not only a specific staining method, but also a special chemical reaction. Temperature is a key factor affecting acid hydrolysis. Insufficient or excessive acid hydrolysis will affect the staining effect of DNA....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/31G05D23/19
CPCG01N1/28G01N1/31G05D23/19
Inventor 黄荣祥余岚岚朱晨雁
Owner MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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