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Stem cell culture process

A stem cell culture and process technology, applied in tissue cell/virus culture devices, non-embryonic pluripotent stem cells, animal cells, etc., can solve the problems of uneven distribution of stem cell solutions, uneven distribution, uneven distribution of stem cells, etc.

Inactive Publication Date: 2022-07-15
周溪桥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When culturing stem cells, the stem cell solution tends to be unevenly distributed on the medium, so that the stem cells will be unevenly distributed in the culture dish during growth, so it is necessary to solve the problem that the stem cell solution is easily unevenly distributed on the medium

Method used

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  • Stem cell culture process

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Experimental program
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Embodiment Construction

[0029] A stem cell culture process, comprising the following steps:

[0030] S1: Take the cell cryotube out of liquid nitrogen, thaw it in a 37°C water bath, and wipe the screw mouth and outer wall of the cryotube with alcohol after taking it out;

[0031] S2: Transfer the cell suspension in the cryopreservation tube to the centrifuge tube of the culture medium, and centrifuge for 4 min;

[0032] S3: After centrifugation, the supernatant was removed by suction, and fresh culture medium was added;

[0033] S4: add the culture medium to the culture device, and then transfer the stem cells in the centrifuge tube to the culture device for culture.

[0034] 75% alcohol was used in the S1.

[0035] The centrifugation speed in the S2 was 1200 rpm.

[0036] like Figure 5-6 As shown, this example can achieve the effect of supporting the petri dish 101 .

[0037] Since the lower side of the culture dish 101 is bonded and connected with the V-shaped piece 105, and the lower end of ...

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Abstract

The invention relates to the field of stem cell culture, in particular to a stem cell culture process. The process comprises the following steps: S1, taking out a cell cryopreservation tube from liquid nitrogen, putting the cell cryopreservation tube into a 37 DEG C water bath for melting, and wiping a spiral opening and an outer wall of the cryopreservation tube with alcohol after taking out the cell cryopreservation tube; s2, transferring the cell suspension in the cryopreservation tube into a centrifugal tube of the culture solution, and centrifuging for 4 minutes; s3, after centrifugation, sucking and removing supernate, and adding a fresh culture solution; and S4, adding a culture solution to the culture device, and then transferring the stem cells in the centrifugal tube to the culture device for culture. The culture device comprises a culture dish, a V-shaped piece and two bottom strips, the lower side of the culture dish is fixedly connected with the V-shaped piece, and the lower end of the V-shaped piece is fixedly connected with the two bottom strips. The stem cells in the centrifugal tube can be transferred to the culture device to be cultured, and the culture device can evenly spread the stem cells.

Description

technical field [0001] The present invention relates to the field of stem cell culture, more specifically to a stem cell culture process. Background technique [0002] Stem cells have the unique ability to self-renew or differentiate into many types of cells in response to appropriate signals. These properties make stem cells have powerful tissue repair, replacement and regeneration capabilities, and human stem cells have attracted much attention in medical research. Embryonic stem cells can differentiate into many more cell types. Various in vivo factors can trigger differentiation, some of which can be replicated in vitro in stem cell culture. When culturing stem cells, the stem cell solution is easily unevenly distributed on the medium, so that the stem cells will be unevenly distributed in the culture dish when growing, so it is necessary to solve the problem that the stem cell solution is easily unevenly distributed on the medium. SUMMARY OF THE INVENTION [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N5/074C12M3/00C12M1/00C12M1/22
CPCC12N5/0606C12M27/16C12M23/10C12M23/48
Inventor 周溪桥
Owner 周溪桥
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