Bioengineered formulations, methods of making and embodiments thereof
A technology of bioengineering and preparation, applied in the direction of medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulations, etc., which can solve problems such as low transparency and rough surfaces, poor integration with host eye tissues, and difficulty in handling, etc. question
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[0145] The present disclosure will now be illustrated using working examples, which are intended to illustrate the workings of the present disclosure and are not intended to imply any limitation on the scope of the present disclosure in a limiting manner. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to the particular methods and experimental conditions described, as such procedures and conditions may apply.
[0146] Materials and methods
[0147] source of stem cells
[0148] For the purposes of this disclosure, sources of stem cells include those derived from, for ex...
example 1
[0159] Bioengineered Formulations
[0160] This example discloses a bioengineered formulation comprising two main components - modified hyaluronic acid and modified collagen peptide. Specifically, in the present disclosure, the modified hyaluronic acid is methacrylated hyaluronic acid (HA-MA), and the modified collagen peptide is a methacrylated recombinant collagen peptide (type I collagen-like peptide or RCP) ( RCP-MA), or thiolated recombinant collagen peptide (RCP-SH). In addition to the aforementioned components, the bioengineered formulation also included a photoinitiator solution (0.001-0.1 mM eosin Y and 0.038 w / v% triethanolamine in phosphate buffered saline (PBS) in PBS, where The components can be photocrosslinked upon short exposure to white light. The bioengineered formulations are solid, transparent hydrogels that adhere strongly to corneal tissue and can be used for noninvasive corneal stromal defects , fast, long-term repair.
[0161] For the purposes of thi...
example 2
[0198] Methods of making bioengineered formulations
[0199] This example describes an optimized method for preparing bioengineered formulations. The steps used to prepare the formulation are Figure 11 is depicted and is also described below:
[0200](i) Vial 1 containing lyophilized bioengineered corneal powder including a combination of 33 kDa HA-MA in a concentration range of 2-7.5 mg and RCP-SH in a concentration range of 5-15 mg / ml and vial II containing photoinitiator solution (100 μl) were heated to root temperature (RT), protected from light.
[0201] (ii) Using a needle and syringe, transfer the photoinitiator solution from vial II to the contents of vial I, protected from light. Vial I was then gently rotated to ensure that the contents were soaked to facilitate dissolution, and then incubated at 37°C for 30 minutes in the dark.
[0202] (iii) Complete dissolution of the components of Vial I to ensure that the final pre-hydrogel formulation is substantially homo...
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Abstract
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