Gene for regulating and controlling rice grain quality and application thereof
A gene and grain technology, applied in the regulation gene of rice grain quality and its application field, can solve the problems of low yield contribution and so on
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Embodiment approach
[0192] As an embodiment of the present invention, the GS10 gene is cloned into a suitable vector by conventional methods, and the recombinant vector carrying the foreign gene is introduced into plant tissues or organs, so that the plant expresses the GS10 gene. Plants overexpressing the GS10 gene can be obtained by regenerating the plant tissues or organs into plants.
[0193] In another aspect, the present invention provides another method for regulating agronomic traits of plants (such as crops), the method comprising: reducing the expression of the GS10 gene in the plant (including making the GS10 gene non-expressed or low-expressed); thereby reducing the Agronomic traits.
[0194] The expression of GS10 gene can be reduced or deleted by various methods well known to those skilled in the art, such as delivering an expression unit (such as an expression vector or virus, etc.) carrying an antisense GS10 gene to a target site, so that cells or plants can be expressed. Tissues...
Embodiment 1
[0209] Example 1, Identification of granulotype-related genes in a recombinant inbred population of W1943 and GLA4
[0210] Common wild rice O. rufipogon and Asian cultivated rice O. sativa L. are the most typical resources for studying rice domestication and evolution in the AA genome of Oryza (Chen et al., 2019). A past polymorphic domestication analysis of 446 wild rice and 950 Asian cultivated rice accessions worldwide identified 55 selective sweeps of natural selection (Huang et al., 2012a). In order to verify and analyze the selective sweep, we used a set of low-abundance sequenced recombinant inbred lines generated by crossing the indica background Guangluai 4 (GLA4) and the partial japonica background wild rice (W1943). A series of QTLs related to grain shape traits were mapped by genome-wide SNP markers ( figure 1 , A). Among these candidate QTLs, GW5 / qSW5 / GSE5, GL7 / GW7 and GL6 were cloned in previous studies, and the one with the highest contribution rate was GW5, ...
Embodiment 2
[0211] Example 2, initial positioning and fine positioning of GS10
[0212] In the population of recombinant inbred line BILs, we initially selected only 16Mb-23.66Mb (IRGSP-1.0) in the background of chromosome 10 through the investigation of grain shape traits (grain length, grain width and aspect ratio). Single fragment replacement line CSSL50 replaced by wild rice W1943. In the BIL population, we combined related molecular markers, mainly insertion and deletion molecular markers (Indel markers), and carried out linkage analysis on traits such as grain length, grain width and aspect ratio combined with WinQTL, etc., and found that there is an indel on chromosome 10. type locus, and had the highest correlation with grain width and thousand-grain weight ( figure 2 ). By observing the agronomic traits related to grain shape in CSSL50 rice, we found that there were significant differences in grain length and grain width between the replacement line and the female parent GLA4 ...
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