Method for delaying passiflora edulia sims spoilage through resuspension and special bacteria thereof

A technology of resuspension and passion fruit, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of musty smell, affecting fruit commerciality, food safety risks, etc., and achieve low production costs , easy to cultivate and preserve and industrialized production, good fresh-keeping effect

Active Publication Date: 2022-07-22
GUIZHOU UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there is still no research showing that Trichoderma harzianum is edible, and there is a certain risk in food safety.
In addition, Trichoderma harzianum will produce a musty smell when it reproduces in large quantities, which will affect the commerciality of the fruit itself.

Method used

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  • Method for delaying passiflora edulia sims spoilage through resuspension and special bacteria thereof
  • Method for delaying passiflora edulia sims spoilage through resuspension and special bacteria thereof
  • Method for delaying passiflora edulia sims spoilage through resuspension and special bacteria thereof

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Embodiment 1

[0042] A method for delaying the spoilage of passion fruit by resuspension, comprising the following:

[0043] (1) Preparation of resuspension:

[0044] a. Separation and purification of R. coronoidum GY, Ecgy (Eurotium cristatum, GY, Ecgy): Weigh 3 g of black hair tea, put it in a 100 mL conical flask containing 50 mL of sterile saline and glass beads, and cultivate at 200 r / min After 60 min, the tea powder suspension was diluted 10 times and 100 times, and 100 μL of the suspension and the dilution were spread on the red Bengal medium for separation. Inverted culture at 26 °C, observed and recorded every day, selected fungal colonies with good growth, and purified with PDA.

[0045] b. Identification of R. coronoides: According to the results of morphological identification of R. coronoides, the DNA of the suspected fungus was extracted and screened, and ITS1 and ITS4 were used to carry out PCR to identify R. coronoidans.

[0046] c. Preparation of R. coronoidi bacterial so...

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Abstract

The method comprises the following steps: culturing eurotium cristatum GY and Ecgy to obtain a culture, grinding 2g of raw dark green tea into powder, adding deionized water at the temperature of 100 DEG C according to a tea-water ratio of 1: 50, extracting for 30 minutes at the speed of 100r / min, filtering by gauze, sterilizing for 30 minutes in an autoclave at the temperature of 121 DEG C under the pressure of 0.2 Mpa, and cooling to room temperature to prepare raw dark green tea soup; re-suspending hyphae obtained after culture filtration and raw dark green tea soup according to the proportion of 1: 1, and uniformly mixing the hyphae and the raw dark green tea soup; and soaking passion fruits in the raw dark green tea soup resuspension for 30-60 seconds, taking out the passion fruits, and naturally storing the passion fruits at room temperature. The passion fruit preservative is high in passion fruit spoilage fungus antagonism, good in passion fruit preservation effect, safe, stable and low in production cost.

Description

technical field [0001] The present invention relates to the technical field of food preservation, in particular to a method for delaying the spoilage of passion fruit by re-suspension, and also to a special bacteria used in the method simultaneously. Background technique [0002] Fruit is a natural nutritious food, containing a variety of nutrients needed for human life. However, fruit production has strong seasonality, regionality and the perishability of the fruit itself, which contradicts the diversity of consumers' needs for fruit and the urgency of off-season adjustment. Taking passion fruit as an example, the current storage and preservation technology is mainly based on low temperature storage, supplemented by 1-Methylcyclopropene (1-Methylcyclopropene, 1-MCP) to delay shrinkage and decay. However, passion fruit is a typical tropical fruit with a more variable breathing type. The above-mentioned storage techniques have limited effect on delaying post-harvest quality ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155C12N1/14C12R1/645
CPCA23B7/155C12N1/14A23V2002/00A23V2200/10
Inventor 董旋王宇曹瑞
Owner GUIZHOU UNIV
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