Preparation method and application of catalase-like g-C3N4/hemin
A technology of catalase and g-c3n4, which is applied in the field of preparation of catalase-like g-C3N4/hemin, can solve the problems of poor catalytic activity, limited applicability, low water solubility and low bioavailability, and achieve Sensitive detection of GSH, simple and safe synthesis conditions, and good application prospects
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[0017] The present invention first provides a kind of catalase-like g-C 3 N 4 The preparation method of / hemin, the method comprises:
[0018] Step 1: Preparation of ultrathin 2D g-C 3 N 4 Nanosheets;
[0019] Step 2: Dissolve hemin in a mixed solvent of methanol and dimethyl sulfoxide under ultrasonic conditions, then dissolve hemin solution with g-C under ultrasonic conditions 3 N 4 The nanosheet solution was mixed, sonicated in a water bath at 0 °C, and then purified by centrifugation. The obtained precipitate was re-dispersed in a buffer solution, continued to be sonicated, and purified by centrifugation. The supernatant was collected and vacuum-dried to obtain catalase-like g-C. 3 N 4 / hemin.
[0020] According to the present invention, the ultrathin two-dimensional g-C is first prepared 3 N 4 Nanosheets, g-C 3 N 4 The preparation method of the nanosheet is a technical method commonly used in the field, and there is no special limitation. Preferably, the NH 4 ...
Embodiment 1
[0026] catalase-like g-C 3 N 4 Preparation of / hemin:
[0027] Step 1: Preparation of ultrathin 2D mesoporous g-C 3 N 4 Nanosheets, g-C 3 N 4 The preparation method of the nanosheet is a technical method commonly used in the field, and there is no special limitation. Preferably, the NH 4 The Cl and dicyandiamide were mixed well and placed in a 50 mL lidded alumina crucible. It was then transferred to a muffle furnace and heated to 550°C at a rate of 5°C / min for 4 hours. The obtained mixture was then cooled to room temperature, taken out and dissolved in a mixed solvent of 25 mL of deionized water and 1 mL of acetic acid, stirred for 4 hours, sonicated for 4 hours, and then the solution was centrifuged. Alternately washed with deionized water and ethanol to remove undispersed large particles, collect the supernatant, and dry at low temperature to obtain ultrathin two-dimensional mesoporous g-C 3 N 4 Nanosheets.
[0028] Step 2: Dissolve 1 g of hemin in 50 mL of mixed ...
Embodiment 2
[0032] catalase-like g-C 3 N 4 / hemin is used for GSH and Cys detection:
[0033] Step 1: Preparation of ultrathin 2D mesoporous g-C 3 N 4 Nanosheets, g-C 3 N 4 The preparation method of the nanosheet is a technical method commonly used in the field, and there is no special limitation. Preferably, the NH 4 The Cl and dicyandiamide were mixed well and placed in a 50 mL lidded alumina crucible. It was then transferred to a muffle furnace and heated to 550°C at a rate of 5°C / min for 4 hours. The obtained mixture was then cooled to room temperature, taken out and dissolved in a mixed solvent of 25 mL of deionized water and 1 mL of acetic acid, stirred for 4 hours, sonicated for 4 hours, and then the solution was centrifuged. Alternately washed with deionized water and ethanol to remove undispersed large particles, collect the supernatant, and dry at low temperature to obtain ultrathin two-dimensional mesoporous g-C 3 N 4 Nanosheets.
[0034] Step 2: Dissolve 1 g of hemin...
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