Hu sheep PLIN2 gene SNP detection method and application of Hu sheep PLIN2 gene SNP detection method in meat quality character early screening

A gene and genome technology, applied in the field of single nucleotide polymorphism sites of Hu sheep PLIN2 gene, can solve difficult problems and achieve the effect of accelerating breeding

Pending Publication Date: 2022-08-05
甘肃润牧生物工程有限责任公司
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Problems solved by technology

However, at present, the PLIN2 gene has been extensively studied mainly at the cellular level induced by fatty acids. There are no related reports on the influence of SNPs in the sheep PLIN2 gene on fatty acid phenotypes. It is still a technical problem to use molecular markers to early screen individuals with beneficial fatty acid phenotypes.

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  • Hu sheep PLIN2 gene SNP detection method and application of Hu sheep PLIN2 gene SNP detection method in meat quality character early screening
  • Hu sheep PLIN2 gene SNP detection method and application of Hu sheep PLIN2 gene SNP detection method in meat quality character early screening
  • Hu sheep PLIN2 gene SNP detection method and application of Hu sheep PLIN2 gene SNP detection method in meat quality character early screening

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Embodiment Construction

[0026] The present invention will be described in further detail below with reference to the accompanying drawings and embodiments. The embodiments are only used to explain the present invention, but not to limit the protection scope of the present invention.

[0027] 1. The SNP locus of Hu sheep PLIN2 gene was obtained by DNA pool sequencing

[0028] S1 sample selection

[0029] From August 2018 to August 2021, a total of 1041 (5 batches) testicles of 6-month-old healthy Hu sheep were collected from Minqin Defu Agricultural Technology Co., Ltd. (Minqin County, Wuwei, Gansu Province) under the same feeding conditions. tissue and longissimus dorsi muscle tissue, and determine the fatty acid composition and content of the longissimus dorsi muscle tissue.

[0030] S2 constructs DNA pool

[0031] a) Use the phenol-chloroform method to extract the genomic DNA of the testis tissue of 1041 Hu sheep individuals, and the specific steps are as follows:

[0032] Ⅰ) Take 10 mg of grou...

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Abstract

The invention discloses a Hu sheep PLIN2 gene SNP (Single Nucleotide Polymorphism) detection method and application of the Hu sheep PLIN2 gene SNP detection method in early screening of meat quality characters. According to the invention, Hu sheep tissue DNA is extracted, an iMLDR technology is used for typing, allele frequency and genotype frequency are calculated, and Hu sheep back longissimus fatty acid percentage and PLIN2 gene mutation site g.287Ggt; correlation analysis is carried out on the site A, the polymorphism of the site and the existing molecular marker obviously related to the percentage of beneficial fatty acid are disclosed, and a basis is provided for selecting and breeding a new sheep strain with excellent meat quality through marker-assisted selection.

Description

technical field [0001] The invention belongs to the field of molecular genetic testing and breeding, and particularly relates to a Hu sheep PLIN2 gene single nucleotide polymorphism (Single nucleotide polymorphism, SNP) site detected by DNA mixed pool sequencing, combined with a fluorescent multiple enzyme ligation reaction ( Improved multiplex ligation detection reaction, iMLDR) technology was used to genotype the locus and use the genotype results to conduct correlation analysis with fatty acid percentage. Background technique [0002] Single nucleotide polymorphism (Single nucleotide polymorphism, SNP) is the most common organism in the process of genetic variation, and is also an important pathway in the process of biological evolution. SNPs occur more frequently in intron regions. SNPs in intron regions do not change the amino acid sequence of the protein encoded by the gene, but may lead to alternative splicing or interact with genetic elements, thereby affecting the t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113C12Q2565/125C12Q2535/122
Inventor 乐祥鹏孔园园张兆才李建栋李宝胜孙锦林宿平平孙万武赵学娣
Owner 甘肃润牧生物工程有限责任公司
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