High-resolution lensless microscope based on RGB LED light source

Abstract

The invention discloses a high-resolution lensless microscope based on an RGB LED light source, which is used for carrying out clear and high-resolution wide-field microscopic imaging on a to-be-detected sample and comprises an imaging system and a computing device. The imaging system comprises an RGB LED light source, a micropore array module, a sample table and an image sensor which are arranged in sequence, and the RGBLED light source is placed at the uppermost part of the whole imaging system; the micropore array module is arranged below the center of the light-emitting surface of the RGB LED light source, and the size of the system is not changed; light emitted by the RGB LED light source passes through the micropore array module to improve the coherence of the light source, and then the light overlaps and illuminates the same area of a sample to be detected on the sample table; a hologram is generated by interference between scattered light and unscattered light of a sample to be measured, the hologram generated by interference under illumination of different wave bands is recorded through an image sensor and transmitted to a computing device, and the computing device eliminates twin images through a multi-wavelength phase recovery algorithm to reconstruct information of the sample to be measured.

Description

technical field [0001] The invention belongs to the technical field of optical imaging, and particularly relates to a high-resolution lensless microscope based on an RGB LED light source. Background technique [0002] On the premise of ensuring the imaging quality, realizing the miniaturization, low cost, and easy operation of wide-field microscopy equipment is a development goal of optical microscopy. The emergence and rapid development of lensless microscopy in recent years provides a promising solution to the above objectives. It abandons expensive and bulky optical lenses, directly attaches the sample to the top of the digital sensor, eliminates the influence of twin images through the corresponding phase recovery algorithm, and realizes the inversion and reconstruction of clear object images. Due to its large field of view, portability, low cost, and deep 3D imaging, it is widely used in large-scale samples such as cervical cancer Pap smear testing, blood smear testing...

AI Technical Summary

Problems solved by technology

This not only sacrifices the compact optical structure of the lensless on-chip digital holographic microscope, but also requires the use of expensive triple narrow-band filters to improve the temporal coherence of the light source due to the larger size of the light source. Reduce system size and cost

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