Methods for hypoxic blood storage and pathogen inactivation

Abstract

The present disclosure relates to a method for reducing pathogens in a blood product comprising: i) removing oxygen from the blood product; ii) reducing blood pathogens t from the blood product: adding an amstamine (S-303) to a final concentration of 0.2 mmol (mM); and glutathione (GSH) to a concentration of 20 mM; iii) reducing S-303 to a concentration below 1 nmol / L, comprising incubating the oxygen reduced blood product under oxygen reduced conditions for up to 6 hours; and to a reduced pathogen, reduced oxygen blood product having an oxygen saturation (sO2) of less than 25%, a pCO2 of 90 mmHg or less at 37 DEG C, and a concentration of aminostatin (S-303) of less than 1 nmol / L.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims priority to US Provisional Application No. 62 / 928,714, filed on October 31, 2019. The entire contents of this application are incorporated herein by reference. technical field [0003] The present disclosure relates to methods of improving the quality and safety of blood and blood products used in transfusion medicine. Background technique [0004] The use of blood and blood components for blood transfusion is a common practice in medicine today, but poses risks to patients due to potential exposure to immunogenic and pathogenic contaminants. This risk is exacerbated by the practice of storing collected blood and blood components for up to several weeks. Whole blood is typically processed by filtration to remove white blood cells (leukopenia) and then centrifuged to separate the 3 major blood components of plasma, platelets and red blood cells. The leukopenic packed red blood cells (LRpRBC) a...

AI Technical Summary

Problems solved by technology

However, recent studies have shown that RBCs obtained from whole blood irradiated using riboflavin / ultraviolet technology (Mirasol, TerumoBCT) are of significantly lower quality compared to untreated study groups, if shelf life under standard storage conditions requires shortened words

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