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Steady-state treatment and processing method of alkaloid in fresh stems and leaves of strobilanthes cusia

A stable, alkaloid technology, applied in pharmaceutical formulations, plant raw materials, medical preparations containing active ingredients, etc., can solve the problem that the yield of indigo is only 0.1% to 0.3%, the biotransformation rate of indole glycosides is low, etc. problems, to avoid excessive local temperature, facilitate directional transformation, and solve the effect of difficult storage

Active Publication Date: 2022-08-09
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the lack of steady-state processing methods for indole glycosides in fresh leaves of indigo, fresh indole leaves can only be naturally fermented in water and beaten with lime to form indigo or indigo paste. The biotransformation rate of glycosides is low, and the yield of indigo is only 0.1% to 0.3%.

Method used

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  • Steady-state treatment and processing method of alkaloid in fresh stems and leaves of strobilanthes cusia
  • Steady-state treatment and processing method of alkaloid in fresh stems and leaves of strobilanthes cusia
  • Steady-state treatment and processing method of alkaloid in fresh stems and leaves of strobilanthes cusia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for stabilizing treatment and processing of alkaloids of fresh stems and leaves of Malanga, comprising the following steps:

[0038] Step 1: Select the raw malan raw materials picked on the same day and stack them in a cool place for 1 day, and then separate the branches and leaves to obtain undamaged leaves, and weigh about 5000g for use;

[0039] Step 2: Withering the Malan leaves, the fresh Malan leaves are withered at room temperature in a rotating drum, the volume ratio of Malan leaves to the rotating drum is 1:5, the rotating speed of the rotating drum is 10 rpm, the wind speed of the fan is 1 m / s, and the air volume is 1000 m 3 / h, withering time 1h;

[0040] Step 3: Microwave drying, pass the withered leaves of Malan wilt through a belt-type microwave automatic distributor, the leaves are stacked with a thickness of 1 cm, and evenly transmitted to the microwave generating area, the microwave frequency is 2.45 GHz, the temperature is 150 ° C, and the lea...

Embodiment 2

[0046] The method for testing the enzyme activity of glycosidase in Malan leaves includes the following steps:

[0047] Step 1: Drawing of the standard curve of p-nitrophenol

[0048] Under certain conditions, β-glucosidase can hydrolyze the glycosidic bond in p-nitrophenyl β-D-glucoside to generate p-nitrophenol, which turns yellow under alkaline conditions, and the yellow substance can be determined by colorimetry The content of β-glucosidase was then calculated.

[0049] Using 1 mol / L sodium carbonate solution as a solvent, the standard solutions of p-nitrophenol with concentrations of 1.73, 3.46, 4.33, 5.12, 6.93 and 10.39 μg / mL were prepared. Take 1 mol / L sodium carbonate solution as blank, scan the maximum absorption peak of this batch of solutions in the ultraviolet-visible region, take the concentration of p-nitrophenol (μg / mL) as the abscissa X, and the absorbance at the maximum absorption peak (A) as the ordinate Y, draw a standard curve.

[0050] Step 2: Preparat...

Embodiment 3

[0058] The measurement of the content of indole glycosides in the dried leaves of Malanga includes the following steps:

[0059] Step 1: Measure the content of indole glycosides by high performance liquid chromatography, and select chromatographic conditions as follows: C18 chromatographic column, methanol:water=20:80, detection wavelength 230nm, column temperature 20°C.

[0060] Step 2: Drawing the standard curve: Precisely weigh 5 mg of indole glycoside standard, add ultrapure water to make up to 10 mL, dissolve by ultrasonic for 30 min, filter with 0.45 μm microporous membrane to obtain filtrate, take 4 μL, 8 μL, 12 μL, 16 μL, 20 μL and 24 μL were injected into the liquid chromatograph, and the regression equation was fitted with the peak area and the corresponding concentration;

[0061] Step 3: Evenly pulverize the prepared dried maltose leaves, weigh 100-200 mg and place them in a 100-mL volumetric flask, add ultrapure water, and sonicate for 30 minutes to fully extract ...

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Abstract

The invention relates to the technical field of plant processing, in particular to a steady-state preparation method of dried strobilanthes cusia leaves with low enzyme activity and high content of indole glycoside and chlorophyll. The invention provides a method for steady-state treatment and processing of alkaloid in fresh stems and leaves of strobilanthes cusia, which comprises the following steps: withering fresh leaves of strobilanthes cusia, carrying out microwave enzyme deactivation treatment, carrying out high-temperature drying, regulating air flow to quickly lose water, quickly cooling to balance moisture, lightly rolling and shaping, and then carrying out microwave low-temperature drying, thereby obtaining the strobilanthes cusia stem and leaf alkaloid. The content of indole glycoside is more than 6%, the enzyme activity of glycosidase is less than 2U / g, the content of chlorophyll is 5-10mg / g, the content of water-soluble substances is more than 30%, and the content of alcohol-soluble substances is more than 15%. The preparation method is clean and pollution-free, and can solve the problem that the strobilanthes cusia is difficult to store, transport and convert after being harvested.

Description

technical field [0001] The invention relates to the technical field of plant processing, and more particularly, the invention relates to a steady-state preparation method of dried Maltia japonica leaves with low enzymatic activity and high content of indole glycosides and chlorophyll. Background technique [0002] Alkaloids from the fresh stems and leaves of Malanga mainly include indoles (indole glycosides, indirubin, indigo, etc.), quinazolones (tryptamine, 4(3H)-quinazolone, etc.) and benzodihydrooxanes Azol-2-one, etc., of which indole glycoside (3-hydroxyindole-β-glucoside) is also called indoside, white needle-like crystals with crystal water, melting point 57-58 ° C, melting point without crystal water It is the precursor of indigo and indirubin, and has anti-virus, anti-tumor, anti-inflammatory and other biological activities. It is an important pharmaceutical intermediate and natural dye raw material for extracts such as indigo, indigo and indirubin. . [0003] In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/195A61K127/00
CPCA61K36/195A61K2236/13A61K2236/17Y02P60/85
Inventor 王成章刘丹阳张昌伟周昊张华兴
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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